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Investigation of a Potential Scintigraphic Tracer for Imaging Apoptosis: Radioiodinated Annexin V-Kunitz Protease Inhibitor Fusion Protein
المؤلفون المشاركون
Yen, Kuo-Chen
Chen, Ming-Wei
Jan, Tong-Rong
Wey, Shiaw-Pyng
Chiang, Chao-Chih
Wun, Tze-Chein
Liao, Mei-Hsiu
Chi, Chin-Wen
Yen, Tzu-Chen
Liao, Tse-Zung
المصدر
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-03-23
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Radiolabeled annexin V (ANV) has been widely used for imaging cell apoptosis.
Recently, a novel ANV-Kunitz-type protease inhibitor fusion protein, ANV-6L15, was found to be a promising probe for improved apoptosis detection based on its higher affinity to phosphatidylserine (PS) compared to native ANV.
The present paper investigates the feasibility of apoptosis detection using radioiodinated ANV-6L15.
Native ANV and ANV-6L15 were labeled with iodine-123 and iodine-125 using Iodogen method.
The binding between the radioiodinated proteins and erythrocyte ghosts or chemical-induced apoptotic cells was examined.
ANV-6L15 can be radioiodinated with high yield (40%−60%) and excellent radiochemical purity (>95%).
123I-ANV-6L15 exhibited a higher binding ratio to erythrocyte ghosts and apoptotic cells compared to 123I-ANV.
The biodistribution of 123I-ANV-6L15 in mice was also characterized.
123I-ANV-6L15 was rapidly cleared from the blood.
High uptake in the liver and the kidneys may limit the evaluation of apoptosis in abdominal regions.
Our data suggest that radiolabled ANV-6L15 may be a better scintigraphic tracer than native ANV for apoptosis detection.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liao, Mei-Hsiu& Jan, Tong-Rong& Chiang, Chao-Chih& Yen, Kuo-Chen& Liao, Tse-Zung& Chen, Ming-Wei…[et al.]. 2011. Investigation of a Potential Scintigraphic Tracer for Imaging Apoptosis: Radioiodinated Annexin V-Kunitz Protease Inhibitor Fusion Protein. BioMed Research International،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-1027624
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liao, Mei-Hsiu…[et al.]. Investigation of a Potential Scintigraphic Tracer for Imaging Apoptosis: Radioiodinated Annexin V-Kunitz Protease Inhibitor Fusion Protein. BioMed Research International No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-1027624
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liao, Mei-Hsiu& Jan, Tong-Rong& Chiang, Chao-Chih& Yen, Kuo-Chen& Liao, Tse-Zung& Chen, Ming-Wei…[et al.]. Investigation of a Potential Scintigraphic Tracer for Imaging Apoptosis: Radioiodinated Annexin V-Kunitz Protease Inhibitor Fusion Protein. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-1027624
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1027624
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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