Amniotic Mesenchymal Stem Cells: A New Source for Hepatocyte-Like Cells and Induction of CFTR Expression by Coculture with Cystic Fibrosis Airway Epithelial Cells
المؤلفون المشاركون
Paracchini, Valentina
Seia, M.
Di Gioia, Sante
Conese, Massimo
Mazzucchelli, Silvia
Colombo, Carla
Colombo, Federico
Degiorgio, Dario
Porretti, Laura
Castellani, Stefano
Carbone, Annalucia
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-10-24
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Cystic fibrosis (CF) is a monogenic disease caused by mutations in the CF transmembrane conductance regulator (CFTR) gene, with lung and liver manifestations.
Because of pitfalls of gene therapy, novel approaches for reconstitution of the airway epithelium and CFTR expression should be explored.
In the present study, human amniotic mesenchymal stem cells (hAMSCs) were isolated from term placentas and characterized for expression of phenotypic and pluripotency markers, and for differentiation potential towards mesoderm (osteogenic and adipogenic) lineages.
Moreover, hAMSCs were induced to differentiate into hepatocyte-like cells, as demonstrated by mixed function oxidase activity and expression of albumin, alpha1-antitrypsin, and CK19.
We also investigated the CFTR expression in hAMSCs upon isolation and in coculture with CF airway epithelial cells.
Freshly isolated hAMSCs displayed low levels of CFTR mRNA, which even decreased with culture passages.
Following staining with the vital dye CM-DiI, hAMSCs were mixed with CFBE41o- respiratory epithelial cells and seeded onto permeable filters.
Flow cytometry demonstrated that 33–50% of hAMSCs acquired a detectable CFTR expression on the apical membrane, a result confirmed by confocal microscopy.
Our data show that amniotic MSCs have the potential to differentiate into epithelial cells of organs relevant in CF pathogenesis and may contribute to partial correction of the CF phenotype.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Paracchini, Valentina& Carbone, Annalucia& Colombo, Federico& Castellani, Stefano& Mazzucchelli, Silvia& Di Gioia, Sante…[et al.]. 2011. Amniotic Mesenchymal Stem Cells: A New Source for Hepatocyte-Like Cells and Induction of CFTR Expression by Coculture with Cystic Fibrosis Airway Epithelial Cells. BioMed Research International،Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-1027925
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Paracchini, Valentina…[et al.]. Amniotic Mesenchymal Stem Cells: A New Source for Hepatocyte-Like Cells and Induction of CFTR Expression by Coculture with Cystic Fibrosis Airway Epithelial Cells. BioMed Research International No. 2012 (2012), pp.1-15.
https://search.emarefa.net/detail/BIM-1027925
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Paracchini, Valentina& Carbone, Annalucia& Colombo, Federico& Castellani, Stefano& Mazzucchelli, Silvia& Di Gioia, Sante…[et al.]. Amniotic Mesenchymal Stem Cells: A New Source for Hepatocyte-Like Cells and Induction of CFTR Expression by Coculture with Cystic Fibrosis Airway Epithelial Cells. BioMed Research International. 2011. Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-1027925
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1027925
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر