Bioassay-Guided Isolation and HPLC Determination of Bioactive Compound That Relate to the Antiplatelet Activity (Adhesion, Secretion, and Aggregation) from Solanum lycopersicum
المؤلفون المشاركون
Fuentes, Eduardo
Palomo, Iván
Astudillo, Luis
Castro, Ricardo
Alarcón, Marcelo
Carrasco, Gilda
Gutiérrez, Margarita
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-11-10
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
In seeking the functionality of foodstuff applicable to medicine, ripe tomato fruits were found to show an antiplatelet activity.
Therefore, the bioactive compound was isolated, structurally identified, and studied for an inhibitory effects on platelet adhesion, secretion, and aggregation.
The concentration of adenosine in ripe tomato fruits (pulp and skin extracts) and its processing by-products (paste and pomace) was determined by reversed-phase high-performance liquid chromatography (HPLC).
According to platelet aggregation inhibition induced by ADP, the total extract residual was fractionated by liquid-liquid separation, obtaining aqueous, ethyl acetate and petroleum ether extracts.
The aqueous extract was subjected to repeated permeation over sephadex LH-20 and semipreparative TLC.
The isolate finally obtained was identified as adenosine on the basis of ESI-MS, 1H NMR, HPLC, and UV spectra.
Adenosine concentration dependently (2.3–457 μM) platelet aggregation inhibited induced by ADP.
Also, adenosine present inhibition of platelet secretion and thrombus formation under flow conditions.
The quantitative HPLC analysis revealed significant amounts of adenosine in ripe tomato fruits and its processing by-products.
From these results, extracts/fractions of ripe tomato fruits and their processing by-products may be referred to as functional food and functional ingredients containing a compound that inhibits platelet function with a potent preventive effect on thrombus formation, as those that occur in stroke.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Fuentes, Eduardo& Castro, Ricardo& Astudillo, Luis& Carrasco, Gilda& Alarcón, Marcelo& Gutiérrez, Margarita…[et al.]. 2012. Bioassay-Guided Isolation and HPLC Determination of Bioactive Compound That Relate to the Antiplatelet Activity (Adhesion, Secretion, and Aggregation) from Solanum lycopersicum. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-1028008
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Fuentes, Eduardo…[et al.]. Bioassay-Guided Isolation and HPLC Determination of Bioactive Compound That Relate to the Antiplatelet Activity (Adhesion, Secretion, and Aggregation) from Solanum lycopersicum. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-1028008
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Fuentes, Eduardo& Castro, Ricardo& Astudillo, Luis& Carrasco, Gilda& Alarcón, Marcelo& Gutiérrez, Margarita…[et al.]. Bioassay-Guided Isolation and HPLC Determination of Bioactive Compound That Relate to the Antiplatelet Activity (Adhesion, Secretion, and Aggregation) from Solanum lycopersicum. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-1028008
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1028008
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر