Inhibition of Hypoxia Inducible Factor Alpha and Astrocyte-Elevated Gene-1 Mediates Cryptotanshinone Exerted Antitumor Activity in Hypoxic PC-3 Cells
المؤلفون المشاركون
Lee, Seok-Geun
Sohn, Eun Jung
Park, Moon Nyeo
Kim, Bonglee
Lee, Hyo-Jeong
Lew, Jae-Hwan
Kim, Hanna Hyun
Kim, Sung-Hoon
Jung, Deok-Beom
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-11-29
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Although cryptotanshinone (CT) was known to exert antitumor activity in several cancers, its molecular mechanism under hypoxia still remains unclear.
Here, the roles of AEG-1 and HIF-1α in CT-induced antitumor activity were investigated in hypoxic PC-3 cells.
CT exerted cytotoxicity against prostate cancer cells and suppressed HIF-1α accumulation and AEG-1 expression in hypoxic PC-3 cells.
Also, AEG-1 was overexpressed in prostate cancer cells.
Interestingly, HIF-1α siRNA transfection enhanced the cleavages of caspase-9,3, and PAPR and decreased expression of Bcl-2 and AEG1 induced by CT in hypoxic PC-3 cells.
Of note, DMOG enhanced the stability of AEG-1 and HIF-1α during hypoxia.
Additionally, CT significantly reduced cellular level of VEGF in PC-3 cells and disturbed tube formation of HUVECs.
Consistently, ChIP assay revealed that CT inhibited the binding of HIF-1α to VEGF promoter.
Furthermore, CT at 10 mg/kg suppressed the growth of PC-3 cells in BALB/c athymic nude mice by 46.4% compared to untreated control.
Consistently, immunohistochemistry revealed decreased expression of Ki-67, CD34, VEGF, carbonic anhydrase IX, and AEG-1 indices in CT-treated group compared to untreated control.
Overall, our findings suggest that CT exerts antitumor activity via inhibition of HIF-1α, AEG1, and VEGF as a potent chemotherapeutic agent.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lee, Hyo-Jeong& Jung, Deok-Beom& Sohn, Eun Jung& Kim, Hanna Hyun& Park, Moon Nyeo& Lew, Jae-Hwan…[et al.]. 2012. Inhibition of Hypoxia Inducible Factor Alpha and Astrocyte-Elevated Gene-1 Mediates Cryptotanshinone Exerted Antitumor Activity in Hypoxic PC-3 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-1028286
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lee, Hyo-Jeong…[et al.]. Inhibition of Hypoxia Inducible Factor Alpha and Astrocyte-Elevated Gene-1 Mediates Cryptotanshinone Exerted Antitumor Activity in Hypoxic PC-3 Cells. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-1028286
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lee, Hyo-Jeong& Jung, Deok-Beom& Sohn, Eun Jung& Kim, Hanna Hyun& Park, Moon Nyeo& Lew, Jae-Hwan…[et al.]. Inhibition of Hypoxia Inducible Factor Alpha and Astrocyte-Elevated Gene-1 Mediates Cryptotanshinone Exerted Antitumor Activity in Hypoxic PC-3 Cells. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-1028286
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1028286
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر