Quantitative Analysis of Sphingomyelin by High-Performance Liquid Chromatography after Enzymatic Hydrolysis
المؤلفون المشاركون
Yoo, Hwan Soo
Yoo, Kwangsik
Lee, Seunghyun
Lee, Youn-Sun
Choi, Kyeong-Mi
Sin, Dong-Mi
Kim, Wonkyun
Lee, Yong-Moon
Hong, Jin-Tae
Yun, Yeo-Pyo
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-06-20
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Sphingomyelin is the most abundant sphingolipid in mammalian cells and is mostly present in the plasma membrane.
A new analytical method using high-performance liquid chromatography (HPLC) was developed to quantify sphingomyelin in mouse plasma and tissues, 3T3-L1 cells, rat aortic smooth muscle cells, and HT-29 cells.
Sphingomyelin and dihydrosphingomyelin, an internal standard, were separated by high-performance thin-layer chromatography and simultaneously hydrolyzed with sphingolipid ceramide N-deacylase and sphingomyelinase to release sphingosine and dihydrosphingosine, respectively.
Sphingomyelin content was measured by HPLC following o-phthalaldehyde derivatization.
Sphingomyelin concentrations in 3T3-L1 cells, rat aortic smooth muscle cells, and HT-29 cells were 60.10±0.24, 62.69±0.08, and 58.38±0.37 pmol/μg protein, respectively, whereas those in brain, kidney, and liver of ICR mice were 55.60±0.43, 43.75±0.21, and 22.26±0.14 pmol/μg protein.
The sphingomyelin concentration in mouse plasma was 407.40±0.31 μM.
The limits of detection and quantification for sphingomyelin were 5 and 20 pmol, respectively, in the HPLC analysis with fluorescence detection.
This sensitivity was sufficient for analyzing sphingomyelin in biological samples.
In conclusion, this analytical method is a sensitive and specific technique for quantifying sphingomyelin and was successfully applied to diverse biological samples with excellent reproducibility.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lee, Seunghyun& Lee, Youn-Sun& Choi, Kyeong-Mi& Yoo, Kwangsik& Sin, Dong-Mi& Kim, Wonkyun…[et al.]. 2012. Quantitative Analysis of Sphingomyelin by High-Performance Liquid Chromatography after Enzymatic Hydrolysis. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-1028288
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lee, Seunghyun…[et al.]. Quantitative Analysis of Sphingomyelin by High-Performance Liquid Chromatography after Enzymatic Hydrolysis. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-1028288
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lee, Seunghyun& Lee, Youn-Sun& Choi, Kyeong-Mi& Yoo, Kwangsik& Sin, Dong-Mi& Kim, Wonkyun…[et al.]. Quantitative Analysis of Sphingomyelin by High-Performance Liquid Chromatography after Enzymatic Hydrolysis. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-1028288
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1028288
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر