Triggering Apoptotic Death of Human Malignant Melanoma A375.S2 Cells by Bufalin: Involvement of Caspase Cascade-Dependent and Independent Mitochondrial Signaling Pathways
المؤلفون المشاركون
Chung, Jing-Gung
Hsiao, Yu-Ping
Yu, Chun-Shu
Yu, Chien-Chih
Huang, Hui-Ying
Tang, Nou-Ying
Yang, Jen-Hung
Huang, An-Cheng
Yang, Jai-Sing
Chiang, Jo-Hua
Lu, Chi-Cheng
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-06-10
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Bufalin was obtained from the skin and parotid venom glands of toad and has been shown to induce cytotoxic effects in various types of cancer cell lines, but there is no report to show that whether bufalin affects human skin cancer cells.
The aim of this investigation was to study the effects of bufalin on human malignant melanoma A375.S2 cells and to elucidate possible mechanisms involved in induction of apoptosis.
A375.S2 cells were treated with different concentrations of bufalin for a specific time period and investigated for effects on apoptotic analyses.
Our results indicated that cells after exposure to bufalin significantly decreased cell viability, and induced cell morphological changes and chromatin condensation in a concentration-dependent manner.
Flow cytometric assays indicated that bufalin promoted ROS productions, loss of mitochondrial membrane potential (ΔΨm), intracellular Ca2+ release, and nitric oxide (NO) formations in A375.S2 cells.
Additionally, the apoptotic induction of bufalin on A375.S2 cells resulted from mitochondrial dysfunction-related responses (disruption of the ΔΨm and releases of cytochrome c, AIF, and Endo G), and activations of caspase-3, caspase-8 and caspase-9 expressions.
Based on those observations, we suggest that bufalin-triggered apoptosis in A375.S2 cells is correlated with extrinsic- and mitochondria-mediated multiple signal pathways.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hsiao, Yu-Ping& Yu, Chun-Shu& Yu, Chien-Chih& Yang, Jai-Sing& Chiang, Jo-Hua& Lu, Chi-Cheng…[et al.]. 2012. Triggering Apoptotic Death of Human Malignant Melanoma A375.S2 Cells by Bufalin: Involvement of Caspase Cascade-Dependent and Independent Mitochondrial Signaling Pathways. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-1028407
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hsiao, Yu-Ping…[et al.]. Triggering Apoptotic Death of Human Malignant Melanoma A375.S2 Cells by Bufalin: Involvement of Caspase Cascade-Dependent and Independent Mitochondrial Signaling Pathways. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-1028407
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hsiao, Yu-Ping& Yu, Chun-Shu& Yu, Chien-Chih& Yang, Jai-Sing& Chiang, Jo-Hua& Lu, Chi-Cheng…[et al.]. Triggering Apoptotic Death of Human Malignant Melanoma A375.S2 Cells by Bufalin: Involvement of Caspase Cascade-Dependent and Independent Mitochondrial Signaling Pathways. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-1028407
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1028407
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر