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Rhynchophylline Protects Cultured Rat Neurons against Methamphetamine Cytotoxicity
المؤلفون المشاركون
Cho, William Chi-Shing
Xu, Dan Dan
Hoeven, Robin
Rong, Rong
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-04-26
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Rhynchophylline (Rhy) is an active component isolated from species of the genus Uncaria which has been used for the treatment of ailments to the central nervous system in traditional Chinese medicine.
Besides acting as a calcium channel blocker, Rhy was also reported to be able to protect against glutamate-induced neuronal death.
We thus hypothesize that Rhy may have neuroprotective activity against methamphetamine (MA).
The primary neurons were cultured directly from the cerebral cortex of neonatal rats, acting as in vitro model in the present study.
The neurotoxicity of MA and the protective effect of Rhy were evaluated by MTT assay.
The effects of MA, Rhy or their combination on intracellular free calcium concentration ([Ca2+]i) were determined in individual neocortical neurons by the Fluo-3/AM tracing method.
The MTT assay demonstrated that MA has a dose-dependent neurotoxicity in neuronal cultures.
The addition of Rhy prior to the exposure to MA prevented neuronal death.
Time course studies with the Fluo-3/AM probe showed that Rhy significantly decreased neuronal [Ca2+]i which was elevated by the exposure to MA.
Our results suggested that Rhy can protect the neuronal cultures against MA exposure and promptly attenuate intracellular calcium overload triggered by MA challenge.
This is the first report demonstrating an inhibitory effect of Rhy against MA impairment in cultured neurons in vitro.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xu, Dan Dan& Hoeven, Robin& Rong, Rong& Cho, William Chi-Shing. 2012. Rhynchophylline Protects Cultured Rat Neurons against Methamphetamine Cytotoxicity. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-1028443
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xu, Dan Dan…[et al.]. Rhynchophylline Protects Cultured Rat Neurons against Methamphetamine Cytotoxicity. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-1028443
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xu, Dan Dan& Hoeven, Robin& Rong, Rong& Cho, William Chi-Shing. Rhynchophylline Protects Cultured Rat Neurons against Methamphetamine Cytotoxicity. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-1028443
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1028443
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