Inhibition of Cell Growth and Induction of Apoptosis by Antrodia camphorata in HER-2neu-Overexpressing Breast Cancer Cells through the Induction of ROS, Depletion of HER-2neu, and Disruption of the PI3KAkt Signaling Pathway
المؤلفون المشاركون
Hseu, You Cheng
Lee, Chuan-Chen
Yang, Hsin-Ling
Way, Tzong-Der
Lin, Kai-Yuan
Juan, Ying-Chen
Cho, Hsin-Ju
Hsu, Li-Sung
Chen, Ssu-Ching
Kumar, K. J. Senthil
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-04-12
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Previously, we demonstrated that a submerged fermentation culture of Antrodia camphorata (AC) promotes cell-cycle arrest and apoptosis in human estrogen receptor-positive/negative breast cancer cells.
However, whether AC is effective against HER-2/neu-overexpressing breast cancers has not been thoroughly elucidated.
In the present study, we showed that AC exhibited a significant cytotoxic effect against HER-2/neu-overexpressing MDA-MB-453 and BT-474 cells.
Immunoblot analysis demonstrated that HER-2/neu and their tyrosine phosphorylation were inhibited by AC in a dose-dependent manner.
An increase in intracellular reactive oxygen species (ROS) was observed in AC-treated cells, whereas antioxidant N-acetylcysteine (NAC) significantly prevented AC induced HER-2/neu depletion and cell death, which directly indicates that AC-induced HER-2/neu depletion and cell death was mediated by ROS generation.
Also, AC significantly downregulated the expression of cyclin D1, cyclin E, and CDK4 followed by the suppression of PI3K/Akt, and their downstream effectors GSK-3β and β-catenin.
Notably, AC-treatment induced apoptotic cell death, which was associated with sub-G1 accumulation, DNA fragmentation, mitochondrial dysfunction, cytochrome c release, caspase-3/-9 activation, PARP degradation, and Bcl-2/Bax dysregulation.
Assays for colony formation also confirmed the growth-inhibitory effects of AC.
This is the first report confirming the anticancer activity of this potentially beneficial mushroom against human HER-2/neu-overexpressing breast cancers.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lee, Chuan-Chen& Yang, Hsin-Ling& Way, Tzong-Der& Kumar, K. J. Senthil& Juan, Ying-Chen& Cho, Hsin-Ju…[et al.]. 2012. Inhibition of Cell Growth and Induction of Apoptosis by Antrodia camphorata in HER-2neu-Overexpressing Breast Cancer Cells through the Induction of ROS, Depletion of HER-2neu, and Disruption of the PI3KAkt Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-1028487
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lee, Chuan-Chen…[et al.]. Inhibition of Cell Growth and Induction of Apoptosis by Antrodia camphorata in HER-2neu-Overexpressing Breast Cancer Cells through the Induction of ROS, Depletion of HER-2neu, and Disruption of the PI3KAkt Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-15.
https://search.emarefa.net/detail/BIM-1028487
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lee, Chuan-Chen& Yang, Hsin-Ling& Way, Tzong-Der& Kumar, K. J. Senthil& Juan, Ying-Chen& Cho, Hsin-Ju…[et al.]. Inhibition of Cell Growth and Induction of Apoptosis by Antrodia camphorata in HER-2neu-Overexpressing Breast Cancer Cells through the Induction of ROS, Depletion of HER-2neu, and Disruption of the PI3KAkt Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-1028487
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1028487
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر