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Effects of the Yangjing Capsule Extract on Steroidogenesis and Apoptosis in Mouse Leydig Cells
المؤلفون المشاركون
Gao, Chao
Cui, Yu-Gui
Sun, Dalin
Jin, Baofang
Zhang, Xindong
Yang, Xiaoyu
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-10-23
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Objectives.
This study aimed to explore the effect and mechanism of Yangjing capsule on testosterone secretion in mouse Leydig tumor cells (MLTC-1).
Methods.
MLTC-1 cells were treated with the Yangjing capsule extract for 24 h.
The testosterone level in medium was measured by radioimmunoassay.
The expression of steroidogenic enzymes (StAR, CYP11A1, and HSD3B) in the cells was examined using real-time RT-PCR and immunoblotting.
Additionally, MLTC-1 cells were treated for 48 h in a serum-free medium.
The cell viability was measured by MTT assay.
The cell cycle and apoptosis were analyzed using flow cytometry.
The expression of activated caspase-3 was analyzed using RT-PCR and a colorimetric protease assay.
Results.
The Yangjing capsule extract increased testosterone production and the expression of StAR, CYP11A1, and HSD3B mRNAs and proteins compared with the control.
H89 significantly inhibited these effects.
The medicine improved the viability of MLTC-1 cells, decreased the number of cells in G0/G1 phase, and increased the number of cells in S-phase, as well as prevented cell apoptosis by inhibiting caspase-3.
Conclusion.
The Yangjing capsule can stimulate MLTC-1 cells to secrete testosterone and may be an alternative treatment for diseases characterized by insufficient testosterone production.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sun, Dalin& Cui, Yu-Gui& Jin, Baofang& Zhang, Xindong& Yang, Xiaoyu& Gao, Chao. 2012. Effects of the Yangjing Capsule Extract on Steroidogenesis and Apoptosis in Mouse Leydig Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-1028675
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sun, Dalin…[et al.]. Effects of the Yangjing Capsule Extract on Steroidogenesis and Apoptosis in Mouse Leydig Cells. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-1028675
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sun, Dalin& Cui, Yu-Gui& Jin, Baofang& Zhang, Xindong& Yang, Xiaoyu& Gao, Chao. Effects of the Yangjing Capsule Extract on Steroidogenesis and Apoptosis in Mouse Leydig Cells. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-1028675
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1028675
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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