![](/images/graphics-bg.png)
A Comparison between Vanadyl, Vanadate, and Decavanadate Effects in Actin Structure and Function: Combination of Several Spectroscopic Studies
المؤلفون المشاركون
Ramos, S.
Aureliano, M.
Moura, José J. G.
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-07-11
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
الملخص EN
The studies about the interaction of actin with vanadium are seldom.
In the present paper the effects of vanadyl, vanadate, and decavanadate in the actin structure and function were compared.
Decavanadate clearly interacts with actin, as shown by V51-NMR spectroscopy.
Decavanadate interaction with actin induces protein cysteine oxidation and vanadyl formation, being both prevented by the natural ligand of the protein, ATP.
Monomeric actin (G-actin) titration with vanadyl, as analysed by EPR spectroscopy, indicates a 1 : 1 binding stoichiometry and a kd of 7.5 μM.
Both decavanadate and vanadyl inhibited G-actin polymerization into actin filaments (F-actin), with a IC50 of 68 and 300 μM, respectively, as analysed by light-scattering assays.
However, only vanadyl induces G-actin intrinsic fluorescence quenching, which suggests the presence of vanadyl high-affinity actin-binding sites.
Decavanadate increases (2.6-fold) actin hydrophobic surface, evaluated using the ANSA probe, whereas vanadyl decreases it (15%).
Finally, both vanadium species increased ε-ATP exchange rate (k=6.5×10−3 and 4.47×10−3 s−1 for decavanadate and vanadyl, resp.).
Putting it all together, it is suggested that actin, which is involved in many cellular processes, might be a potential target not only for decavanadate but above all for vanadyl.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ramos, S.& Moura, José J. G.& Aureliano, M.. 2012. A Comparison between Vanadyl, Vanadate, and Decavanadate Effects in Actin Structure and Function: Combination of Several Spectroscopic Studies. Journal of Spectroscopy،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-1029357
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ramos, S.…[et al.]. A Comparison between Vanadyl, Vanadate, and Decavanadate Effects in Actin Structure and Function: Combination of Several Spectroscopic Studies. Journal of Spectroscopy No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-1029357
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ramos, S.& Moura, José J. G.& Aureliano, M.. A Comparison between Vanadyl, Vanadate, and Decavanadate Effects in Actin Structure and Function: Combination of Several Spectroscopic Studies. Journal of Spectroscopy. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-1029357
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1029357
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)