Heart Cells with Regenerative Potential from Pediatric Patients with End Stage Heart Failure: A Translatable Method to Enrich and Propagate

المؤلفون المشاركون

Jacobs, Jeffrey P.
Steele, Ann
Boucek, Robert J.
Steele, Peter
Asante-Korang, Alfred
Chamizo, Wilfredo
Steele, Jasmine
Chai, Paul J.
Quintessenza, James A.

المصدر

Stem Cells International

العدد

المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-8، 8ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2012-08-14

دولة النشر

مصر

عدد الصفحات

8

الملخص EN

Background.

Human cardiac-derived progenitor cells (hCPCs) have shown promise in treating heart failure (HF) in adults.

The purpose of this study was to describe derivation of hCPCs from pediatric patients with end-stage HF.

Methods.

At surgery, discarded right atrial tissues (hAA) were obtained from HF patients (n=25; hAA-CHF).

Minced tissues were suspended in complete (serum-containing) DMEM.

Cells were selected for their tissue migration and expression of stem cell factor receptor (hc-kit).

Characterization of hc-kitpositive cells included immunohistochemical screening with a panel of monoclonal antibodies.

Results.

Cells, including phase-bright cells identified as hc-kitpositive, spontaneously emigrated from hAA-CHF in suspended explant cultures (SEC) after Day 7.

When cocultured with tissue, emigrated hc-kitpositive cells proliferated, first as loosely attached clones and later as multicellular clusters.

At Day 21~5% of cells were hc-kitpositive.

Between Days 14 and 28 hc-kitpositive cells exhibited mesodermal commitment (GATA-4positive and NKX2.5positive); then after Day 28 cardiac lineages (flk-1positive, smooth muscle actinpositive, troponin-Ipositive, and myosin light chainpositive).

Conclusions.

C-kitpositive hCPCs can be derived from atrial tissue of pediatric patients with end-stage HF.

SEC is a novel culture method for derivation of migratory hc-kitpositive cells that favors clinical translation by reducing the need for exogenously added factors to expand hCPCs in vitro.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Steele, Ann& Boucek, Robert J.& Jacobs, Jeffrey P.& Steele, Peter& Asante-Korang, Alfred& Chamizo, Wilfredo…[et al.]. 2012. Heart Cells with Regenerative Potential from Pediatric Patients with End Stage Heart Failure: A Translatable Method to Enrich and Propagate. Stem Cells International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-1030022

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Steele, Ann…[et al.]. Heart Cells with Regenerative Potential from Pediatric Patients with End Stage Heart Failure: A Translatable Method to Enrich and Propagate. Stem Cells International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-1030022

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Steele, Ann& Boucek, Robert J.& Jacobs, Jeffrey P.& Steele, Peter& Asante-Korang, Alfred& Chamizo, Wilfredo…[et al.]. Heart Cells with Regenerative Potential from Pediatric Patients with End Stage Heart Failure: A Translatable Method to Enrich and Propagate. Stem Cells International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-1030022

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1030022