Heart Cells with Regenerative Potential from Pediatric Patients with End Stage Heart Failure: A Translatable Method to Enrich and Propagate
المؤلفون المشاركون
Jacobs, Jeffrey P.
Steele, Ann
Boucek, Robert J.
Steele, Peter
Asante-Korang, Alfred
Chamizo, Wilfredo
Steele, Jasmine
Chai, Paul J.
Quintessenza, James A.
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-08-14
دولة النشر
مصر
عدد الصفحات
8
الملخص EN
Background.
Human cardiac-derived progenitor cells (hCPCs) have shown promise in treating heart failure (HF) in adults.
The purpose of this study was to describe derivation of hCPCs from pediatric patients with end-stage HF.
Methods.
At surgery, discarded right atrial tissues (hAA) were obtained from HF patients (n=25; hAA-CHF).
Minced tissues were suspended in complete (serum-containing) DMEM.
Cells were selected for their tissue migration and expression of stem cell factor receptor (hc-kit).
Characterization of hc-kitpositive cells included immunohistochemical screening with a panel of monoclonal antibodies.
Results.
Cells, including phase-bright cells identified as hc-kitpositive, spontaneously emigrated from hAA-CHF in suspended explant cultures (SEC) after Day 7.
When cocultured with tissue, emigrated hc-kitpositive cells proliferated, first as loosely attached clones and later as multicellular clusters.
At Day 21~5% of cells were hc-kitpositive.
Between Days 14 and 28 hc-kitpositive cells exhibited mesodermal commitment (GATA-4positive and NKX2.5positive); then after Day 28 cardiac lineages (flk-1positive, smooth muscle actinpositive, troponin-Ipositive, and myosin light chainpositive).
Conclusions.
C-kitpositive hCPCs can be derived from atrial tissue of pediatric patients with end-stage HF.
SEC is a novel culture method for derivation of migratory hc-kitpositive cells that favors clinical translation by reducing the need for exogenously added factors to expand hCPCs in vitro.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Steele, Ann& Boucek, Robert J.& Jacobs, Jeffrey P.& Steele, Peter& Asante-Korang, Alfred& Chamizo, Wilfredo…[et al.]. 2012. Heart Cells with Regenerative Potential from Pediatric Patients with End Stage Heart Failure: A Translatable Method to Enrich and Propagate. Stem Cells International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-1030022
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Steele, Ann…[et al.]. Heart Cells with Regenerative Potential from Pediatric Patients with End Stage Heart Failure: A Translatable Method to Enrich and Propagate. Stem Cells International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-1030022
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Steele, Ann& Boucek, Robert J.& Jacobs, Jeffrey P.& Steele, Peter& Asante-Korang, Alfred& Chamizo, Wilfredo…[et al.]. Heart Cells with Regenerative Potential from Pediatric Patients with End Stage Heart Failure: A Translatable Method to Enrich and Propagate. Stem Cells International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-1030022
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1030022
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر