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Chemical Modifications of PhTX-I Myotoxin from Porthidium hyoprora Snake Venom: Effects on Structural, Enzymatic, and Pharmacological Properties
المؤلفون المشاركون
Corrêa, Daniel H. A.
Hollanda, Luciana M.
Lancellotti, Marcelo
Ramos, C.
Huancahuire-Vega, Salomón
Ponce Soto, L. A.
Marangoni, Sérgio
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-12-19
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
We recently described the isolation of a basic PLA2 (PhTX-I) from Porthidium hyoprora snake venom.
This toxin exhibits high catalytic activity, induces in vivo myotoxicity, moderates footpad edema, and causes in vitro neuromuscular blockade.
Here, we describe the chemical modifications of specific amino acid residues (His, Tyr, Lys, and Trp), performed in PhTX-I, to study their effects on the structural, enzymatic, and pharmacological properties of this myotoxin.
After chemical treatment, a single His, 4 Tyr, 7 Lys, and one Trp residues were modified.
The secondary structure of the protein remained unchanged as measured by circular dichroism; however other results indicated the critical role played by Lys and Tyr residues in myotoxic, neurotoxic activities and mainly in the cytotoxicity displayed by PhTX-I.
His residue and therefore catalytic activity of PhTX-I are relevant for edematogenic, neurotoxic, and myotoxic effects, but not for its cytotoxic activity.
This dissociation observed between enzymatic activity and some pharmacological effects suggests that other molecular regions distinct from the catalytic site may also play a role in the toxic activities exerted by this myotoxin.
Our observations supported the hypothesis that both the catalytic sites as the hypothetical pharmacological sites are relevant to the pharmacological profile of PhTX-I.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Huancahuire-Vega, Salomón& Corrêa, Daniel H. A.& Hollanda, Luciana M.& Lancellotti, Marcelo& Ramos, C.& Ponce Soto, L. A.…[et al.]. 2012. Chemical Modifications of PhTX-I Myotoxin from Porthidium hyoprora Snake Venom: Effects on Structural, Enzymatic, and Pharmacological Properties. BioMed Research International،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1030073
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Huancahuire-Vega, Salomón…[et al.]. Chemical Modifications of PhTX-I Myotoxin from Porthidium hyoprora Snake Venom: Effects on Structural, Enzymatic, and Pharmacological Properties. BioMed Research International No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-1030073
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Huancahuire-Vega, Salomón& Corrêa, Daniel H. A.& Hollanda, Luciana M.& Lancellotti, Marcelo& Ramos, C.& Ponce Soto, L. A.…[et al.]. Chemical Modifications of PhTX-I Myotoxin from Porthidium hyoprora Snake Venom: Effects on Structural, Enzymatic, and Pharmacological Properties. BioMed Research International. 2012. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1030073
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1030073
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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