![](/images/graphics-bg.png)
Total 25-Hydroxyvitamin D Determination by an Entry Level Triple Quadrupole Instrument: Comparison between Two Commercial Kits
المؤلفون المشاركون
Persichilli, Silvia
Cocci, Andrea
Gervasoni, Jacopo
Zuppi, Cecilia
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-02-11
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
الملخص EN
Objective.
25-hydroxyvitamin D2/D3 (25-OHD2/D3) determination is a reliable biomarker for vitamin D status.
Liquid chromatography-tandem mass spectrometry was recently proposed as a reference method for vitamin D status evaluation.
The aim of this work is to compare two commercial kits (Chromsystems and PerkinElmer) for 25-OHD2/D3 determination by our entry level LC-MS/MS.
Design and Methods.
Chromsystems kit adds an online trap column to an HPLC column and provides atmospheric pressure chemical ionization, isotopically labeled internal standard, and 4 calibrator points.
PerkinElmer kit uses a solvent extraction and protein precipitation method.
This kit can be used with or without derivatization with, respectively, electrospray and atmospheric pressure chemical ionization.
For each analyte, there are isotopically labeled internal standards and 7 deuterated calibrator points.
Results.
Performance characteristics are acceptable for both methods.
Mean bias between methods calculated on 70 samples was 1.9 ng/mL.
Linear regression analysis gave an R2 of 0.94.
25-OHD2 is detectable only with PerkinElmer kit in derivatized assay option.
Conclusion.
Both methods are suitable for routine.
Chromsystems kit minimizes manual sample preparation, requiring only protein precipitation, but, with our system, 25-OHD2 is not detectable.
PerkinElmer kit without derivatization does not guarantee acceptable performance with our LC-MS/MS system, as sample is not purified online.
Derivatization provides sufficient sensitivity for 25-OHD2 detection.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gervasoni, Jacopo& Cocci, Andrea& Zuppi, Cecilia& Persichilli, Silvia. 2013. Total 25-Hydroxyvitamin D Determination by an Entry Level Triple Quadrupole Instrument: Comparison between Two Commercial Kits. BioMed Research International،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-1030267
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gervasoni, Jacopo…[et al.]. Total 25-Hydroxyvitamin D Determination by an Entry Level Triple Quadrupole Instrument: Comparison between Two Commercial Kits. BioMed Research International No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-1030267
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gervasoni, Jacopo& Cocci, Andrea& Zuppi, Cecilia& Persichilli, Silvia. Total 25-Hydroxyvitamin D Determination by an Entry Level Triple Quadrupole Instrument: Comparison between Two Commercial Kits. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-1030267
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1030267
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)