Frataxin mRNA Isoforms in FRDA Patients and Normal Subjects: Effect of Tocotrienol Supplementation
المؤلفون المشاركون
Abruzzo, Provvidenza Maria
Marini, Marina
Bolotta, Alessandra
Malisardi, Gemma
Manfredini, Stefano
Ghezzo, Alessandro
Pini, Antonella
Tasco, Gianluca
Casadio, Rita
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-09-23
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Friedreich’s ataxia (FRDA) is caused by deficient expression of the mitochondrial protein frataxin involved in the formation of iron-sulphur complexes and by consequent oxidative stress.
We analysed low-dose tocotrienol supplementation effects on the expression of the three splice variant isoforms (FXN-1, FXN-2, and FXN-3) in mononuclear blood cells of FRDA patients and healthy subjects.
In FRDA patients, tocotrienol leads to a specific and significant increase of FXN-3 expression while not affecting FXN-1 and FXN-2 expression.
Since no structural and functional details were available for FNX-2 and FXN-3, 3D models were built.
FXN-1, the canonical isoform, was then docked on the human iron-sulphur complex, and functional interactions were computed; when FXN-1 was replaced by FXN-2 or FNX-3, we found that the interactions were maintained, thus suggesting a possible biological role for both isoforms in human cells.
Finally, in order to evaluate whether tocotrienol enhancement of FXN-3 was mediated by an increase in peroxisome proliferator-activated receptor-γ (PPARG), PPARG expression was evaluated.
At a low dose of tocotrienol, the increase of FXN-3 expression appeared to be independent of PPARG expression.
Our data show that it is possible to modulate the mRNA expression of the minor frataxin isoforms and that they may have a functional role.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Abruzzo, Provvidenza Maria& Marini, Marina& Bolotta, Alessandra& Malisardi, Gemma& Manfredini, Stefano& Ghezzo, Alessandro…[et al.]. 2013. Frataxin mRNA Isoforms in FRDA Patients and Normal Subjects: Effect of Tocotrienol Supplementation. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030274
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Abruzzo, Provvidenza Maria…[et al.]. Frataxin mRNA Isoforms in FRDA Patients and Normal Subjects: Effect of Tocotrienol Supplementation. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1030274
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Abruzzo, Provvidenza Maria& Marini, Marina& Bolotta, Alessandra& Malisardi, Gemma& Manfredini, Stefano& Ghezzo, Alessandro…[et al.]. Frataxin mRNA Isoforms in FRDA Patients and Normal Subjects: Effect of Tocotrienol Supplementation. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030274
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1030274
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر