Role of Rheum Polysaccharide in the Cytokines Produced by Peripheral Blood Monocytes in TLR4 Mediated HLA-B27 Associated AAU
المؤلفون المشاركون
Hu, Xiaofeng
Liu, Xuhui
Zhang, Xiaosheng
Li, Zhongqiu
Lu, Hong
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-4، 4ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-09-16
دولة النشر
مصر
عدد الصفحات
4
التخصصات الرئيسية
الملخص EN
Purpose.
To evaluate the effect of a traditional Chinese medicine, Rheum Polysaccharide (RP), on the in vitro production of tumor necrosis factor alpha (TNF-α) and interleukin-10 (IL-10) by lipopolysaccharide- (LPS-)stimulated human monocytes from HLA-B27 associated acute anterior uveitis patients of convalescence stage.
Method.
PBMC samples were isolated from 10 HLA-B27 associated acute anterior uveitis, incubated, respectively, and divided into 4 groups as follows: (1) controls, PBS was added in final concentration of 1 mg·L−1, (2) stimulated by LPS, LPS was added in final concentration of 1 mg·L−1, (3) stimulated by LPS + HTA125, 30 minutes before the adding of LPS in final concentration of 1 mg·L−1, the final concentration of 5 mg·L−1 of the HTA125 was added, and (4) stimulated by LPS + RP, 30 minutes before the adding of LPS in final concentration 1 mg·L−1, the final concentration 100 mg·L−1 of the RP was added.
Supernatants were used to quantify the amounts of TNF-α and IL-10 released in time course using enzyme-linked immunosorbent assay (ELISA).
Result.
After stimulated by lps, the concentrations of TNF-α and IL-10 in culture supernatants of patients are significantly higher than control group at all time points (P<0.01).
Blockage of TLR-4 by HTA125 can decrease the production of TNF-α and IL-10 compared with lps group (P<0.01, except at 4 h group of IL-10).
Concentration of TNF-α and IL-10 also decreases in the LPS + RP group (P<0.01) but not so significantly as in the LPS + HTA125 group.
Conclusion.
As anti-TLR4 monoclonal antibodies, rheum Polysaccharide can also inhibit the secretion of cytokines produced by monocytes from HLA-B27 positive AAU patients of convalescence stage.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Xuhui& Hu, Xiaofeng& Zhang, Xiaosheng& Li, Zhongqiu& Lu, Hong. 2013. Role of Rheum Polysaccharide in the Cytokines Produced by Peripheral Blood Monocytes in TLR4 Mediated HLA-B27 Associated AAU. BioMed Research International،Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-1030545
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Xuhui…[et al.]. Role of Rheum Polysaccharide in the Cytokines Produced by Peripheral Blood Monocytes in TLR4 Mediated HLA-B27 Associated AAU. BioMed Research International No. 2013 (2013), pp.1-4.
https://search.emarefa.net/detail/BIM-1030545
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Xuhui& Hu, Xiaofeng& Zhang, Xiaosheng& Li, Zhongqiu& Lu, Hong. Role of Rheum Polysaccharide in the Cytokines Produced by Peripheral Blood Monocytes in TLR4 Mediated HLA-B27 Associated AAU. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-1030545
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1030545
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر