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Genetic and Biochemical Diversity of Paenibacillus larvae Isolated from Tunisian Infected Honey Bee Broods
المؤلفون المشاركون
Cherif, Ameur
Boudabous, Abdelatif
Crotti, Elena
Hamdi, Chadlia
Essanaa, Jihène
Sansonno, Luigi
Abdi, Khaoula
Barbouche, Naima
Balloi, Annalisa
Gonella, Elena
Alma, Alberto
Daffonchio, Daniele
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-09-02
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Paenibacillus larvae is the causative agent of American foulbrood (AFB), a virulent disease of honeybee (Apis mellifera) larvae.
In Tunisia, AFB has been detected in many beekeeping areas, where it causes important economic losses, but nothing is known about the diversity of the causing agent.
Seventy-five isolates of P.
larvae, identified by biochemical tests and 16S rRNA gene sequencing, were obtained from fifteen contaminated broods showing typical AFB symptoms, collected in different locations in the northern part of the country.
Using BOX-PCR, a distinct profile of P.
larvae with respect to related Paenibacillus species was detected which may be useful for its identification.
Some P.
larvae-specific bands represented novel potential molecular markers for the species.
BOX-PCR fingerprints indicated a relatively high intraspecific diversity among the isolates not described previously with several molecular polymorphisms identifying six genotypes on polyacrylamide gel.
Polymorphisms were also detected in several biochemical characters (indol production, nitrate reduction, and methyl red and oxidase tests).
Contrary to the relatively high intraspecies molecular and phenotypic diversity, the in vivo virulence of three selected P.
larvae genotypes did not differ significantly, suggesting that the genotypic/phenotypic differences are neutral or related to ecological aspects other than virulence.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hamdi, Chadlia& Essanaa, Jihène& Sansonno, Luigi& Crotti, Elena& Abdi, Khaoula& Barbouche, Naima…[et al.]. 2013. Genetic and Biochemical Diversity of Paenibacillus larvae Isolated from Tunisian Infected Honey Bee Broods. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030580
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hamdi, Chadlia…[et al.]. Genetic and Biochemical Diversity of Paenibacillus larvae Isolated from Tunisian Infected Honey Bee Broods. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1030580
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hamdi, Chadlia& Essanaa, Jihène& Sansonno, Luigi& Crotti, Elena& Abdi, Khaoula& Barbouche, Naima…[et al.]. Genetic and Biochemical Diversity of Paenibacillus larvae Isolated from Tunisian Infected Honey Bee Broods. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1030580
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1030580
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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