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Biochemical, Pharmacological, and Structural Characterization of New Basic PLA2 Bbil-TX from Bothriopsis bilineata Snake Venom
المؤلفون المشاركون
Corasolla Carregari, Victor
Stuani Floriano, Rafael
Rodrigues-Simioni, Lea
Winck, Flavia V.
Ponce Soto, L. A.
Baldasso, P. A.
Marangoni, Sérgio
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-09-30
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Bbil-TX, a PLA2, was purified from Bothriopsis bilineata snake venom after only one chromatographic step using RP-HPLC on μ-Bondapak C-18 column.
A molecular mass of 14243.8 Da was confirmed by Q-Tof Ultima API ESI/MS (TOF MS mode) mass spectrometry.
The partial protein sequence obtained was then submitted to BLASTp, with the search restricted to PLA2 from snakes and shows high identity values when compared to other PLA2s.
PLA2 activity was presented in the presence of a synthetic substrate and showed a minimum sigmoidal behavior, reaching its maximal activity at pH 8.0 and 25–37∘C.
Maximum PLA2 activity required Ca2+ and in the presence of Cd2+, Zn2+, Mn2+, and Mg2+ it was reduced in the presence or absence of Ca2+.
Crotapotin from Crotalus durissus cascavella rattlesnake venom and antihemorrhagic factor DA2-II from Didelphis albiventris opossum sera under optimal conditions significantly inhibit the enzymatic activity.
Bbil-TX induces myonecrosis in mice.
The fraction does not show a significant cytotoxic activity in myotubes and myoblasts (C2C12).
The inflammatory events induced in the serum of mice by Bbil-TX isolated from Bothriopsis bilineata snake venom were investigated.
An increase in vascular permeability and in the levels of TNF-a, IL-6, and IL-1 was was induced.
Since Bbil-TX exerts a stronger proinflammatory effect, the phospholipid hydrolysis may be relevant for these phenomena.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Corasolla Carregari, Victor& Stuani Floriano, Rafael& Rodrigues-Simioni, Lea& Winck, Flavia V.& Baldasso, P. A.& Ponce Soto, L. A.…[et al.]. 2012. Biochemical, Pharmacological, and Structural Characterization of New Basic PLA2 Bbil-TX from Bothriopsis bilineata Snake Venom. BioMed Research International،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1030719
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Corasolla Carregari, Victor…[et al.]. Biochemical, Pharmacological, and Structural Characterization of New Basic PLA2 Bbil-TX from Bothriopsis bilineata Snake Venom. BioMed Research International No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-1030719
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Corasolla Carregari, Victor& Stuani Floriano, Rafael& Rodrigues-Simioni, Lea& Winck, Flavia V.& Baldasso, P. A.& Ponce Soto, L. A.…[et al.]. Biochemical, Pharmacological, and Structural Characterization of New Basic PLA2 Bbil-TX from Bothriopsis bilineata Snake Venom. BioMed Research International. 2012. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1030719
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1030719
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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