Levels of Key Enzymes of Methionine-Homocysteine Metabolism in Preeclampsia
المؤلفون المشاركون
Rice, Gregory E.
Pérez-Sepúlveda, Alejandra
E. Illanes, Sebastian
España-Perrot, Pedro P.
Fernández B, Ximena
Ahumada, Verónica
Bustos, Vicente
Arraztoa, José Antonio
Dobierzewska, Aneta
Figueroa-Diesel, Horacio
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-08-20
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Objective.
To evaluate the role of key enzymes in the methionine-homocysteine metabolism (MHM) in the physiopathology of preeclampsia (PE).
Methods.
Plasma and placenta from pregnant women (32 controls and 16 PE patients) were analyzed after informed consent.
Protein was quantified by western blot.
RNA was obtained with RNA purification kit and was quantified by reverse transcritase followed by real-time PCR (RT-qPCR).
Identification of the C677T and A1298C methylenetetrahydrofolate reductase (MTHFR) single-nucleotide polymorphisms (SNPs) and A2756G methionine synthase (MTR) SNP was performed using PCR followed by a high-resolution melting (HRM) analysis.
S-adenosyl methionine (SAM) and S-adenosyl homocysteine (SAH) were measured in plasma using high-performance liquid chromatography-tandem mass spectrometry (HPLC/MS/MS).
The SNP association analysis was carried out using Fisher’s exact test.
Statistical analysis was performed using a Mann-Whitney test.
Results.
RNA expression of MTHFR and MTR was significantly higher in patients with PE as compared with controls.
Protein, SAM, and SAH levels showed no significant difference between preeclamptic patients and controls.
No statistical differences between controls and PE patients were observed with the different SNPs studied.
Conclusion.
The RNA expression of MTHFR and MTR is elevated in placentas of PE patients, highlighting a potential compensation mechanism of the methionine-homocysteine metabolism in the physiopathology of this disease.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Pérez-Sepúlveda, Alejandra& España-Perrot, Pedro P.& Fernández B, Ximena& Ahumada, Verónica& Bustos, Vicente& Arraztoa, José Antonio…[et al.]. 2013. Levels of Key Enzymes of Methionine-Homocysteine Metabolism in Preeclampsia. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1030857
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Pérez-Sepúlveda, Alejandra…[et al.]. Levels of Key Enzymes of Methionine-Homocysteine Metabolism in Preeclampsia. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1030857
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Pérez-Sepúlveda, Alejandra& España-Perrot, Pedro P.& Fernández B, Ximena& Ahumada, Verónica& Bustos, Vicente& Arraztoa, José Antonio…[et al.]. Levels of Key Enzymes of Methionine-Homocysteine Metabolism in Preeclampsia. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1030857
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1030857
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر