Evaluation of Multiplex PCR with Enhanced Spore Germination for Detection of Clostridium difficile from Stool Samples of the Hospitalized Patients
المؤلفون المشاركون
Chankhamhaengdecha, Surang
Janvilisri, Tavan
Hadpanus, Piyapong
Aroonnual, Amornrat
Ngamwongsatit, Puriya
Chotiprasitsakul, Darunee
Chongtrakool, Piriyaporn
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-03-17
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Clostridium difficile poses as the most common etiologic agent of nosocomial diarrhea.
Although there are many diagnostic methods to detect C.
difficile directly from stool samples, the nucleic acid-based approach has been largely performed in several laboratories due to its high sensitivity and specificity as well as rapid turnaround time.
In this study, a multiplex PCR was newly designed with recent accumulated nucleotide sequences.
The PCR testing with various C.
difficile ribotypes, other Clostridium spp., and non-Clostridium strains revealed 100% specificity with the ability to detect as low as ~22 genomic copy number per PCR reaction.
Different combinations of sample processing were evaluated prior to multiplex PCR for the detection of C.
difficile in fecal samples from hospitalized patients.
The most optimal condition was the non-selective enrichment at 37∘C for 1 h in brain heart infusion broth supplemented with taurocholate, followed by the multiplex PCR.
The detection limit after sample processing was shown as being 5 spores per gram of fecal sample.
Two hundred and thirty-eight fecal samples collected from the University affiliated hospital were analyzed by the enrichment multiplex PCR procedure.
The results suggested that the combination of sample processing with the high-performance detection method would be applicable for routine diagnostic use in clinical setting.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chankhamhaengdecha, Surang& Hadpanus, Piyapong& Aroonnual, Amornrat& Ngamwongsatit, Puriya& Chotiprasitsakul, Darunee& Chongtrakool, Piriyaporn…[et al.]. 2013. Evaluation of Multiplex PCR with Enhanced Spore Germination for Detection of Clostridium difficile from Stool Samples of the Hospitalized Patients. BioMed Research International،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1031049
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chankhamhaengdecha, Surang…[et al.]. Evaluation of Multiplex PCR with Enhanced Spore Germination for Detection of Clostridium difficile from Stool Samples of the Hospitalized Patients. BioMed Research International No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1031049
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chankhamhaengdecha, Surang& Hadpanus, Piyapong& Aroonnual, Amornrat& Ngamwongsatit, Puriya& Chotiprasitsakul, Darunee& Chongtrakool, Piriyaporn…[et al.]. Evaluation of Multiplex PCR with Enhanced Spore Germination for Detection of Clostridium difficile from Stool Samples of the Hospitalized Patients. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1031049
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1031049
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر