Myogenic Differentiation of Mesenchymal Stem Cells in a Newly Developed Neurotised AV-Loop Model
المؤلفون المشاركون
Horch, Raymund E.
Kneser, Ulrich
Bitto, Franz F.
Klumpp, Dorothee
Lange, Claudia
Boos, Anja M.
Arkudas, Andreas
Bleiziffer, Oliver
Beier, Justus P.
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-09-10
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Generation of axially vascularized muscle tissue constitutes a promising new approach to restoration of damaged muscle tissue.
Mesenchymal stemcells (MSC), with their ability to be expanded to large cell numbers without losing their differentiation capacity into the myogenic lineage, could offer a promising cell source to generate neomuscle tissue.
In vitro experiments showed that cocultures of primary myoblasts and MSC undergo myogenic differentiation by stimulation with bFGF and dexamethasone.
A newly developed AV-Loop model with neurotization was established in this study.
It encompasses axial vascularization and the additional implantation of a motor nerve serving as myogenic stimulator.
Myoblasts and MSCs were coimplantated in a prevascularized isolation chamber.
Cells were differentiated by addition of bFGF and dexamethasone plus implantation of a motor nerve.
After 8 weeks, we could observe areas of myogenic differentiation with α-sarcomeric actin and MHC expression in the constructs.
Quantitative PCR analysis showed an expression of myogenic markers in all specimens.
Thus, neurotization and addition of bFGF and dexamethasone allow myogenic differentiation of MSC in an axially vascularized in vivo model for the first time.
These findings are a new step towards clinical applicability of skeletal muscle tissue engineering and display its potential for regenerative medicine.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bitto, Franz F.& Klumpp, Dorothee& Lange, Claudia& Boos, Anja M.& Arkudas, Andreas& Bleiziffer, Oliver…[et al.]. 2013. Myogenic Differentiation of Mesenchymal Stem Cells in a Newly Developed Neurotised AV-Loop Model. BioMed Research International،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1031117
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bitto, Franz F.…[et al.]. Myogenic Differentiation of Mesenchymal Stem Cells in a Newly Developed Neurotised AV-Loop Model. BioMed Research International No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-1031117
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bitto, Franz F.& Klumpp, Dorothee& Lange, Claudia& Boos, Anja M.& Arkudas, Andreas& Bleiziffer, Oliver…[et al.]. Myogenic Differentiation of Mesenchymal Stem Cells in a Newly Developed Neurotised AV-Loop Model. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1031117
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1031117
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر