Role of Toll-Like Receptor 4 on Lupus Lung Injury and Atherosclerosis in LPS-Challenge ApoE−− Mice
المؤلفون المشاركون
Wang, Zhenhua
Lin, Ling
Xu, Dongming
Huang, Ziyang
Ouyang, Qiufang
Lu, Huixia
Zhang, Yun
Lin, Huili
Ni, Jing-qin
Chen, Xiaoqing
المصدر
Journal of Immunology Research
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-09-15
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
To investigate the pathologic mechanisms of toll-like receptor 4 (TLR4) in lung injury and atherosclerosis, ApoE−/− or wild-type mice were intraperitoneally administered saline, lipopolysaccharides (LPS), or LPS plus TAK-242 (TLR4 inhibitor), respectively, twice a week for 4 weeks.
Serum autoantibody of antinuclear antibody (ANA), anti-double-stranded DNA (anti-dsDNA), and cytokines of interferon-gamma (IFN-γ), tumor necrosis factor (TNF-α), and interleukin-1 (IL-1β) were assessed by ELISA.
Hematoxylin and eosin (HE) and Perl's stains for lung pathomorphology as well as HE staining for atherosclerosis were employed.
TLR4 in macrophages was detected by double immunofluorescent staining.
While protein expressions of TLR4, nuclear factor-kappa B p65 (NF-κB p65), and B cell activating factor belonging to the TNF family (BAFF) were examined by immunohistochemistry.
We found that serum autoantibody (ANA and anti-dsDNA), cytokines (IFN-γ, TNF-α, IL-1β), lung inflammation, and intima-media thickness in brachiocephalic artery were obviously increased after LPS challenge in both genotypes, but to a lesser extent in wild-type strains.
And those alterations were alleviated by coadministration of LPS and TAK-242.
Mechanistically, upregulation of TLR4, NF-κb, and BAFF was involved.
We concluded that TLR4/NF-κb/BAFF in macrophages might be a possible common autoimmune pathway that caused lung injury and atherosclerosis.
TLR4 signal will be a therapeutic target in atherosclerosis and immune-mediated lung injury.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ni, Jing-qin& Ouyang, Qiufang& Lin, Ling& Huang, Ziyang& Lu, Huixia& Chen, Xiaoqing…[et al.]. 2013. Role of Toll-Like Receptor 4 on Lupus Lung Injury and Atherosclerosis in LPS-Challenge ApoE−− Mice. Journal of Immunology Research،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1031281
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ni, Jing-qin…[et al.]. Role of Toll-Like Receptor 4 on Lupus Lung Injury and Atherosclerosis in LPS-Challenge ApoE−− Mice. Journal of Immunology Research No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-1031281
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ni, Jing-qin& Ouyang, Qiufang& Lin, Ling& Huang, Ziyang& Lu, Huixia& Chen, Xiaoqing…[et al.]. Role of Toll-Like Receptor 4 on Lupus Lung Injury and Atherosclerosis in LPS-Challenge ApoE−− Mice. Journal of Immunology Research. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1031281
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1031281
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر