Cationic Gelatin Nanoparticles for Drug Delivery to the Ocular Surface: In Vitro and In Vivo Evaluation
المؤلفون المشاركون
Tseng, Ching-Li
Chen, Ko-Hua
Lee, Yen-Hsien
Wu, Chi-Chang
Su, Wen-Yu
Lin, Fen-Huei
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-12-31
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
To develop an effective ocular drug delivery carrier, we prepared two different charged gelatin nanoparticles (GPs) and evaluated particle size, surface charge, and morphology.
The in vitro biocompatibility of GPs was assessed using human corneal epithelium (HCE) cells and in vivo safety by administering them as eye drops to New Zealand rabbits.
The GPs prepared using type A gelatin were positively charged (GP(+), +33 mV; size, 180.6±45.7 nm).
Water-soluble tetrazolium salt (WST)-1 assay showed that both GPs were nontoxic to HCE cells.
The fluorescence intensity of HCE cells cultured with cationic GPs conjugated with a fluorescent dye was higher than that of the anionic GP-treated HCE cells.
In vivo examination showed no serious irritation to the rabbit eyes.
Furthermore, corneal thickness and ocular pressure in the eyes of the treated rabbits were similar to those in the eyes of normal rabbits.
Microscopic examination of corneal cryosections showed widely distributed fluorescent nanocarriers, from the anterior to the posterior part of the cornea of the GP(+) group, and higher fluorescence intensity in the GP(+) group was also observed.
In conclusion, GPs as cationic colloidal carriers were efficiently adsorbed on the negatively charged cornea without irritating the eyes of the rabbits and can be retained in the cornea for a longer time.
Thus, GPs(+) have a great potential as vehicles for ocular drug delivery.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tseng, Ching-Li& Chen, Ko-Hua& Su, Wen-Yu& Lee, Yen-Hsien& Wu, Chi-Chang& Lin, Fen-Huei. 2013. Cationic Gelatin Nanoparticles for Drug Delivery to the Ocular Surface: In Vitro and In Vivo Evaluation. Journal of Nanomaterials،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1031320
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tseng, Ching-Li…[et al.]. Cationic Gelatin Nanoparticles for Drug Delivery to the Ocular Surface: In Vitro and In Vivo Evaluation. Journal of Nanomaterials No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-1031320
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tseng, Ching-Li& Chen, Ko-Hua& Su, Wen-Yu& Lee, Yen-Hsien& Wu, Chi-Chang& Lin, Fen-Huei. Cationic Gelatin Nanoparticles for Drug Delivery to the Ocular Surface: In Vitro and In Vivo Evaluation. Journal of Nanomaterials. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1031320
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1031320
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر