Intranuclear Crosstalk between Extracellular Regulated Kinase12 and Signal Transducer and Activator of Transcription 3 Regulates JEG-3 Choriocarcinoma Cell Invasion and Proliferation
المؤلفون المشاركون
Weber, Maja
Schleussner, Ekkehard
Markert, Udo R.
Morales-Prieto, Diana M.
Ospina-Prieto, Stephanie
Chaiwangyen, Wittaya
Hölters, Sebastian
Fitzgerald, Justine S.
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-10-30
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الطب البشري
تكنولوجيا المعلومات وعلم الحاسوب
الملخص EN
Invasiveness of trophoblast and choriocarcinoma cells is in part mediated via leukemia inhibitory factor- (LIF-) induced activation of signal transducer and activator of transcription 3 (STAT3).
The regulation of STAT3 phosphorylation at its ser727 binding site, possible crosstalk with intracellular MAPK signaling, and their functional implications are the object of the present investigation.
JEG-3 choriocarcinoma cells were cultured in presence/absence of LIF and the specific ERK1/2 inhibitor (U0126).
Phosphorylation of signaling molecules (p-STAT3 (ser727 and tyr705) and p-ERK1/2 (thr 202/tyr 204)) was assessed per Western blot.
Immunocytochemistry confirmed results, but also pinpointed the location of phosphorylated signaling molecules.
STAT3 DNA-binding capacity was studied with a colorimetric ELISA-based assay.
Cell viability and invasion capability were assessed by MTS and Matrigel assays.
Our results demonstrate that LIF-induced phosphorylation of STAT3 (tyr705 and ser727) is significantly increased after blocking ERK1/2.
STAT3 DNA-binding capacity and cell invasiveness are enhanced after LIF stimulation and ERK1/2 blockage.
In contrast, proliferation is enhanced by LIF but reduced after ERK1/2 inhibition.
The findings herein show that blocking ERK1/2 increases LIF-induced STAT3 phosphorylation and STAT3 DNA-binding capacity by an intranuclear crosstalk, which leads to enhanced invasiveness and reduced proliferation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Morales-Prieto, Diana M.& Ospina-Prieto, Stephanie& Chaiwangyen, Wittaya& Weber, Maja& Hölters, Sebastian& Schleussner, Ekkehard…[et al.]. 2013. Intranuclear Crosstalk between Extracellular Regulated Kinase12 and Signal Transducer and Activator of Transcription 3 Regulates JEG-3 Choriocarcinoma Cell Invasion and Proliferation. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1032710
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Morales-Prieto, Diana M.…[et al.]. Intranuclear Crosstalk between Extracellular Regulated Kinase12 and Signal Transducer and Activator of Transcription 3 Regulates JEG-3 Choriocarcinoma Cell Invasion and Proliferation. The Scientific World Journal No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1032710
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Morales-Prieto, Diana M.& Ospina-Prieto, Stephanie& Chaiwangyen, Wittaya& Weber, Maja& Hölters, Sebastian& Schleussner, Ekkehard…[et al.]. Intranuclear Crosstalk between Extracellular Regulated Kinase12 and Signal Transducer and Activator of Transcription 3 Regulates JEG-3 Choriocarcinoma Cell Invasion and Proliferation. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1032710
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1032710
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر