Chondrocyte Differentiation of Human Endometrial Gland-Derived MSCs in Layered Cell Sheets
المؤلفون المشاركون
Shimizu, Tatsuya
Sekine, Waki
Umezawa, Akihiro
Yamato, Masayuki
Haraguchi, Yuji
Okano, Teruo
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-11-18
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الطب البشري
تكنولوجيا المعلومات وعلم الحاسوب
الملخص EN
Recently, regenerative medicine using engineered three-dimensional (3D) tissues has been focused.
In the fields of cell therapy and regenerative medicine, mesenchymal stem cells (MSCs) are attractive autologous cell sources.
While, in bioengineered tissues, a 3D environment may affect the differentiation of the stem cells, little is known regarding the effect of 3D environment on cellular differentiation.
In this study, MSC differentiation in in vitro 3D tissue models was assessed by human endometrial gland-derived MSCs (hEMSCs) and cell sheet technology.
hEMSC sheets were layered into cell-dense 3D tissues and were cultured on porous membranes.
The tissue sections revealed that chondrocyte-like cells were found within the multilayered cell sheets even at 24 h after layering.
Immunostainings of chondrospecific markers were positive within those cell sheet constructs.
In addition, sulfated glycosaminoglycan accumulation within the tissues increased in proportion to the numbers of layered cell sheets.
The findings suggested that a high cell density and hypoxic environment in 3D tissues by layering cell sheets might accelerate a rapid differentiation of hEMSCs into chondrocytes without the help of chondro-differentiation reagents.
These tissue models using cell sheets would give new insights to stem cell differentiation in 3D environment and contribute to the future application of stem cells to cartilage regenerative therapy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sekine, Waki& Haraguchi, Yuji& Shimizu, Tatsuya& Yamato, Masayuki& Umezawa, Akihiro& Okano, Teruo. 2013. Chondrocyte Differentiation of Human Endometrial Gland-Derived MSCs in Layered Cell Sheets. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1032814
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sekine, Waki…[et al.]. Chondrocyte Differentiation of Human Endometrial Gland-Derived MSCs in Layered Cell Sheets. The Scientific World Journal No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1032814
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sekine, Waki& Haraguchi, Yuji& Shimizu, Tatsuya& Yamato, Masayuki& Umezawa, Akihiro& Okano, Teruo. Chondrocyte Differentiation of Human Endometrial Gland-Derived MSCs in Layered Cell Sheets. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1032814
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1032814
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر