Use of Tissue Culture Techniques for Producing Virus-Free Plant in Garlic and Their Identification through Real-Time PCR
المؤلفون المشاركون
Baktemur, Gökhan
Taşkın, Hatıra
Büyükalaca, Saadet
Kurul, Mehmet
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-07-07
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
الطب البشري
تكنولوجيا المعلومات وعلم الحاسوب
الملخص EN
This study was performed for comparison of meristem culture technique with shoot tip culture technique for obtaining virus-free plant, comparison of micropropagation success of two different nutrient media, and determination of effectiveness of real-time PCR assay for the detection of viruses.
Two different garlic species (Allium sativum and Allium tuncelianum) and two different nutrient media were used in this experiment.
Results showed that Medium 2 was more successful compared to Medium 1 for both A.
tuncelianum and A.
sativum (Kastamonu garlic clone).
In vitro plants obtained via meristem and shoot tip cultures were tested for determination of onion yellow dwarf virus (OYDV) and leek yellow stripe virus (LYSV) through real-time PCR assay.
In garlic plants propagated via meristem culture, we could not detect any virus.
OYDV and LYSV viruses were detected in plants obtained via shoot tip culture.
OYDV virus was observed in amount of 80% and 73% of tested plants for A.
tuncelianum and A.
sativum, respectively.
LYSV virus was found in amount of 67% of tested plants of A.
tuncelianum and in amount of 87% of tested plants of A.
sativum in this study.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Taşkın, Hatıra& Baktemur, Gökhan& Kurul, Mehmet& Büyükalaca, Saadet. 2013. Use of Tissue Culture Techniques for Producing Virus-Free Plant in Garlic and Their Identification through Real-Time PCR. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-1033295
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Taşkın, Hatıra…[et al.]. Use of Tissue Culture Techniques for Producing Virus-Free Plant in Garlic and Their Identification through Real-Time PCR. The Scientific World Journal No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-1033295
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Taşkın, Hatıra& Baktemur, Gökhan& Kurul, Mehmet& Büyükalaca, Saadet. Use of Tissue Culture Techniques for Producing Virus-Free Plant in Garlic and Their Identification through Real-Time PCR. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-1033295
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1033295
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر