Phytochemical and Antibacterial Investigations of the Extracts and Fractions from the Stem Bark of Hymenaea stigonocarpa Mart. ex Hayne and Effect on Ultrastructure of Staphylococcus aureus Induced by Hydroalcoholic Extract
المؤلفون المشاركون
Dimech, Gustavo Santiago
de Oliveira, Anne Gabrielle Vasconcelos
Carvalho, Maria da Conceição
Ximenes, Eulália Azevedo
Ferreira, Magda Rhayanny Assunção
Soares, Luiz Alberto Lira
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-12-14
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الطب البشري
تكنولوجيا المعلومات وعلم الحاسوب
الملخص EN
The aim of this study was to investigate the antimicrobial activity of different extracts and fractions obtained from Hymenaea stigonocarpa stem barks.
The cyclohexanic, ethyl acetate, ethanol, aqueous, and hydroalcoholic extracts were obtained by maceration.
The hydroalcoholic extract was partitioned, which resulted in the ethyl acetate and aqueous fractions.
All extracts and fractions were subjected to phytochemical screening and evaluation of total phenol and tannin contents.
An HPLC-DAD and ultrastructural alterations analysis were performed.
Terpenes and coumarins were detected in the cyclohexanic extract.
Flavonoids and condensed tannins were present in the other extracts and fractions.
The extracts with the highest contents of tannins, ethanol (EE), hydroalcoholic (HE), and aqueous fraction (AF) showed also the highest antimicrobial activity.
The MIC values ranged from 64 to 526 µg/mL.
The chromatographic fingerprints suggest the presence of astilbin and other flavonoids in EE and HE.
Presence of the thick cell wall, undulating outer layer, abnormal septa, and leakage of the cytoplasmic contents and absence of cell wall and cell lyses were the main alterations observed on Staphylococcus aureus ATCC 33591 after treatment with the Hymenaea stigonocarpa hydroalcoholic extract.
The presence of phenolic compounds like flavonoids and tannins is possibly the reason for the antimicrobial activity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Dimech, Gustavo Santiago& Soares, Luiz Alberto Lira& Ferreira, Magda Rhayanny Assunção& de Oliveira, Anne Gabrielle Vasconcelos& Carvalho, Maria da Conceição& Ximenes, Eulália Azevedo. 2013. Phytochemical and Antibacterial Investigations of the Extracts and Fractions from the Stem Bark of Hymenaea stigonocarpa Mart. ex Hayne and Effect on Ultrastructure of Staphylococcus aureus Induced by Hydroalcoholic Extract. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1033383
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Dimech, Gustavo Santiago…[et al.]. Phytochemical and Antibacterial Investigations of the Extracts and Fractions from the Stem Bark of Hymenaea stigonocarpa Mart. ex Hayne and Effect on Ultrastructure of Staphylococcus aureus Induced by Hydroalcoholic Extract. The Scientific World Journal No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1033383
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Dimech, Gustavo Santiago& Soares, Luiz Alberto Lira& Ferreira, Magda Rhayanny Assunção& de Oliveira, Anne Gabrielle Vasconcelos& Carvalho, Maria da Conceição& Ximenes, Eulália Azevedo. Phytochemical and Antibacterial Investigations of the Extracts and Fractions from the Stem Bark of Hymenaea stigonocarpa Mart. ex Hayne and Effect on Ultrastructure of Staphylococcus aureus Induced by Hydroalcoholic Extract. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1033383
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1033383
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر