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Microfluidic Method of Pig Oocyte Quality Assessment in relation to Different Follicular Size Based on Lab-on-Chip Technology
المؤلفون المشاركون
Nowicki, Michał
Zabel, Maciej
Kempisty, Bartosz
Walczak, Rafał
Antosik, Paweł
Sniadek, Patrycja
Rybska, Marta
Piotrowska, Hanna
Dziuban, Jan
Jaśkowski, Jędrzej M.
Bukowska, Dorota
Brüssow, Klaus P.
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-06-09
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Since microfollicular environment and the size of the follicle are important markers influencing oocyte quality, the aim of this study is to present the spectral characterization of oocytes isolated from follicles of various sizes using lab-on-chip (LOC) technology and to demonstrate how follicle size may affect oocyte quality.
Porcine oocytes (each, n = 100 ) recovered from follicles of different sizes, for example, from large (>5 mm), medium (3–5 mm), and small (<3 mm), were analyzed after preceding in vitro maturation (IVM).
The LOC analysis was performed using a silicon-glass sandwich with two glass optical fibers positioned “face-to-face.” Oocytes collected from follicles of different size classes revealed specific and distinguishable spectral characteristics.
The absorbance spectra (microspectrometric specificity) for oocytes isolated from large, medium, and small follicles differ significantly ( P < 0.05 ) and the absorbance wavelengths were between 626 and 628 nm, between 618 and 620 nm, and less than 618 nm, respectively.
The present study offers a parametric and objective method of porcine oocyte assessment.
However, up to now this study has been used to evidence spectral markers associated with follicular size in pigs, only.
Further investigations with functional-biological assays and comparing LOC analyses with fertilization and pregnancy success and the outcome of healthy offspring must be performed.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kempisty, Bartosz& Walczak, Rafał& Antosik, Paweł& Sniadek, Patrycja& Rybska, Marta& Piotrowska, Hanna…[et al.]. 2014. Microfluidic Method of Pig Oocyte Quality Assessment in relation to Different Follicular Size Based on Lab-on-Chip Technology. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1034469
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kempisty, Bartosz…[et al.]. Microfluidic Method of Pig Oocyte Quality Assessment in relation to Different Follicular Size Based on Lab-on-Chip Technology. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1034469
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kempisty, Bartosz& Walczak, Rafał& Antosik, Paweł& Sniadek, Patrycja& Rybska, Marta& Piotrowska, Hanna…[et al.]. Microfluidic Method of Pig Oocyte Quality Assessment in relation to Different Follicular Size Based on Lab-on-Chip Technology. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1034469
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1034469
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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