Preparation and Characterization of Gelatin-Based Mucoadhesive Nanocomposites as Intravesical Gene Delivery Scaffolds
المؤلفون المشاركون
Liu, Ching-Wen
Lin, Kai-Jen
Tsai, Ching-Chung
Tsai, Tong-Rong
Yu, Tsan-Jung
Chang, Li-Ching
Wang, Hao-Kuang
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-12-14
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
This study aimed to develop optimal gelatin-based mucoadhesive nanocomposites as scaffolds for intravesical gene delivery to the urothelium.
Hydrogels were prepared by chemically crosslinking gelatin A or B with glutaraldehyde.
Physicochemical and delivery properties including hydration ratio, viscosity, size, yield, thermosensitivity, and enzymatic degradation were studied, and scanning electron microscopy (SEM) was carried out.
The optimal hydrogels (H), composed of 15% gelatin A175, displayed an 81.5% yield rate, 87.1% hydration ratio, 42.9 Pa·s viscosity, and 125.8 nm particle size.
The crosslinking density of the hydrogels was determined by performing pronase degradation and ninhydrin assays.
In vitro lentivirus (LV) release studies involving p24 capsid protein analysis in 293T cells revealed that hydrogels containing lentivirus (H-LV) had a higher cumulative release than that observed for LV alone (3.7-, 2.3-, and 2.3-fold at days 1, 3, and 5, resp.).
Lentivirus from lentivector constructed green fluorescent protein (GFP) was then entrapped in hydrogels (H-LV-GFP).
H-LV-GFP showed enhanced gene delivery in AY-27 cells in vitro and to rat urothelium by intravesical instillation in vivo.
Cystometrogram showed mucoadhesive H-LV reduced peak micturition and threshold pressure and increased bladder compliance.
In this study, we successfully developed first optimal gelatin-based mucoadhesive nanocomposites as intravesical gene delivery scaffolds.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Ching-Wen& Chang, Li-Ching& Lin, Kai-Jen& Yu, Tsan-Jung& Tsai, Ching-Chung& Wang, Hao-Kuang…[et al.]. 2014. Preparation and Characterization of Gelatin-Based Mucoadhesive Nanocomposites as Intravesical Gene Delivery Scaffolds. BioMed Research International،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1034472
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Ching-Wen…[et al.]. Preparation and Characterization of Gelatin-Based Mucoadhesive Nanocomposites as Intravesical Gene Delivery Scaffolds. BioMed Research International No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1034472
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Ching-Wen& Chang, Li-Ching& Lin, Kai-Jen& Yu, Tsan-Jung& Tsai, Ching-Chung& Wang, Hao-Kuang…[et al.]. Preparation and Characterization of Gelatin-Based Mucoadhesive Nanocomposites as Intravesical Gene Delivery Scaffolds. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1034472
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1034472
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر