Establishment of Cell Lines from Both Myeloma Bone Marrow and Plasmacytoma: SNU_MM1393_BM and SNU_MM1393_SC from a Single Patient
المؤلفون المشاركون
Jung, Woo June
Koh, Youngil
Ahn, Kwang-Sung
Yoon, Sung-Soo
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-08-12
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Purpose.
We tried to establish clinically relevant human myeloma cell lines that can contribute to the understanding of multiple myeloma (MM).
Materials and Methods.
Mononuclear cells obtained from MM patient’s bone marrow were injected via tail vein in an NRG/SCID mouse.
Fourteen weeks after the injection, tumor developed at subcutis of the mouse.
The engraftment of MM cells into mouse bone marrow (BM) was also observed.
We separated and cultured cells from subcutis and BM.
Results.
After the separation and culture of cells from subcutis and BM, we established two cell lines originating from a single patient (SNU_MM1393_BM and SNU_MM1393_SC).
Karyotype of the two newly established MM cell lines showed tetraploidy which is different from the karyotype of the patient (diploidy) indicating clonal evolution.
In contrast to SNU_MM1393_BM, cell proliferation of SNU_MM1393_SC was IL-6 independent.
SNU_MM1393_BM and SNU_MM1393_SC showed high degree of resistance against bortezomib compared to U266 cell line.
SNU_MM1393_BM had the greater lethality compared to SNU_MM1393_SC.
Conclusion.
Two cell lines harboring different site tropisms established from a single patient showed differences in cytokine response and lethality.
Our newly established cell lines could be used as a tool to understand the biology of multiple myeloma.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Koh, Youngil& Jung, Woo June& Ahn, Kwang-Sung& Yoon, Sung-Soo. 2014. Establishment of Cell Lines from Both Myeloma Bone Marrow and Plasmacytoma: SNU_MM1393_BM and SNU_MM1393_SC from a Single Patient. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1034484
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Koh, Youngil…[et al.]. Establishment of Cell Lines from Both Myeloma Bone Marrow and Plasmacytoma: SNU_MM1393_BM and SNU_MM1393_SC from a Single Patient. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1034484
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Koh, Youngil& Jung, Woo June& Ahn, Kwang-Sung& Yoon, Sung-Soo. Establishment of Cell Lines from Both Myeloma Bone Marrow and Plasmacytoma: SNU_MM1393_BM and SNU_MM1393_SC from a Single Patient. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1034484
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1034484
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر