Inhibition of Peroxisome Proliferator-Activated Receptor Gamma Prevents the Melanogenesis in Murine B16F10 Melanoma Cells
المؤلفون المشاركون
Chen, Pei-Ru
Chuang, Yun-Ju
Chen, Jiun-Han
Chang, Junn-Liang
Tang, Shih-Tsang
Pan, Shwu-Fen
Lin, Tzer-Bin
Chen, Kang-Hua
Chen, Mei-Jung
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-08-28
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The purpose of this study was to investigate if PPAR γ plays a role in the melanogenesis.
B16/F10 cells were divided into five groups: control, melanin stimulating hormone ( α -MSH), α -MSH+retinol, α -MSH+GW9662 (PPAR γ antagonist), and GW9662.
Cells in the control group were cultured in the Dulbecco’s modified Eagle’s medium (DMEM) for 48 hrs.
To initiate the melanogenesis, cells in all α -MSH groups were cultured in medium containing α -MSH (10 nM) for 48 hrs.
Cells were treated simultaneously with retinol (5 μM) in the α -MSH+retinol group.
Instead of retinol, GW9662 (10 μM) was cocultured in the α -MSH+GW9662 group.
Cells in the final group were cultured in the DMEM with GW9662.
All the analyses were carried out 48 hours after treatments.
The α -MSH was able to increase cell number, melanin production, and the activity of tyrosinase, the limiting enzyme in melanogenesis.
These α -MSH-induced changes were prevented either by retinol or by GW9662.
Further analyses of the activities of antioxidant enzymes including glutathione, catalase, and the superoxide dismutase (SOD) showed that α -MSH treatment raised the activity of SOD which was dependent on PPAR γ level.
According to our results, the α -MSH-induced melanogenesis was PPAR γ dependent, which also modulated the expression of SOD.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Jiun-Han& Chang, Junn-Liang& Chen, Pei-Ru& Chuang, Yun-Ju& Tang, Shih-Tsang& Pan, Shwu-Fen…[et al.]. 2014. Inhibition of Peroxisome Proliferator-Activated Receptor Gamma Prevents the Melanogenesis in Murine B16F10 Melanoma Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1034542
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Jiun-Han…[et al.]. Inhibition of Peroxisome Proliferator-Activated Receptor Gamma Prevents the Melanogenesis in Murine B16F10 Melanoma Cells. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1034542
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Jiun-Han& Chang, Junn-Liang& Chen, Pei-Ru& Chuang, Yun-Ju& Tang, Shih-Tsang& Pan, Shwu-Fen…[et al.]. Inhibition of Peroxisome Proliferator-Activated Receptor Gamma Prevents the Melanogenesis in Murine B16F10 Melanoma Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1034542
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1034542
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر