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Molecular Identification and Quantification of Tetracycline and Erythromycin Resistance Genes in Spanish and Italian Retail Cheeses
المؤلفون المشاركون
Torriani, S.
Flórez, Ana Belén
Alegría, Ángel
Rossi, Franca
Delgado, Susana
Felis, Giovanna E.
Mayo, Baltasar
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-09-11
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Large antibiotic resistance gene pools in the microbiota of foods may ultimately pose a risk for human health.
This study reports the identification and quantification of tetracycline- and erythromycin-resistant populations, resistance genes, and gene diversity in traditional Spanish and Italian cheeses, via culturing, conventional PCR, real-time quantitative PCR (qPCR), and denaturing gradient gel electrophoresis (DGGE).
The numbers of resistant bacteria varied widely among the antibiotics and the different cheese varieties; in some cheeses, all the bacterial populations seemed to be resistant.
Up to eight antibiotic resistance genes were sought by gene-specific PCR, six with respect to tetracycline, that is, tet(K), tet(L), tet(M), tet(O), tet(S), and tet(W), and two with respect to erythromycin, that is, erm(B) and erm(F).
The most common resistance genes in the analysed cheeses were tet(S), tet(W), tet(M), and erm(B).
The copy numbers of these genes, as quantified by qPCR, ranged widely between cheeses (from 4.94 to 10.18log10/g).
DGGE analysis revealed distinct banding profiles and two polymorphic nucleotide positions for tet(W)-carrying cheeses, though the similarity of the sequences suggests this tet(W) to have a monophyletic origin.
Traditional cheeses would therefore appear to act as reservoirs for large numbers of many types of antibiotic resistance determinants.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Flórez, Ana Belén& Alegría, Ángel& Rossi, Franca& Delgado, Susana& Felis, Giovanna E.& Torriani, S.…[et al.]. 2014. Molecular Identification and Quantification of Tetracycline and Erythromycin Resistance Genes in Spanish and Italian Retail Cheeses. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1034555
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Flórez, Ana Belén…[et al.]. Molecular Identification and Quantification of Tetracycline and Erythromycin Resistance Genes in Spanish and Italian Retail Cheeses. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1034555
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Flórez, Ana Belén& Alegría, Ángel& Rossi, Franca& Delgado, Susana& Felis, Giovanna E.& Torriani, S.…[et al.]. Molecular Identification and Quantification of Tetracycline and Erythromycin Resistance Genes in Spanish and Italian Retail Cheeses. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1034555
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1034555
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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