A Higher Frequency of CD4+CXCR5+ T Follicular Helper Cells in Adult Patients with Minimal Change Disease

الملخص EN

Background.

T follicular helper (TFH) cells are involved in the humoral immune responses.

This study is aimed at examining the frequencies of different subsets of CD4+CXCR5+ TFH cells in adult patients with minimal change disease (MCD).

Methods.

A total of 27 patients and 14 healthy controls (HC) were characterized for the levels of sera cytokines, inducible T-cell costimulator (ICOS), and programmed death 1 (PD-1) of positive TFH cells by flow cytometry.

The level of sera IL-21 was examined; 24 h urinary protein and eGFR were calculated.

The potential correlation between the frequency of different subsets of TFH cells and the values of clinical measures in MCD patients were analyzed.

Results.

The frequency of circulating CD4+CXCR5+, CD4+CXCR5+ICOS+, and CD4+CXCR5+PD-1+ TFH cells and the levels of sera IL-17A, IFN-γ, IL-2, IL-10, IL-4, and IL-21 were significantly higher in MCD patients ( P < 0.05 ) than that in the HC group.

Furthermore, the percentages of circulating CD4+CXCR5+ TFH cells were negatively correlated with the values of eGFR ( r = - 0.4849 , P < 0.05 ) and the percentages of CD4+CXCR5+PD-1+ TFH cells were correlated positively with the levels of serum IL-21 ( r = 0.6137 , P < 0.05 ) and 24 h urinary protein ( r = 0.1410 , P < 0.05 ) in those patients.

Also, the percentages of CD4+CXCR5+ICOS+ TFH cells were correlated positively with the levels of serum IL-21 ( r = 0.6201 , P < 0.05 ) and 24 h urinary protein ( r = 0.7519 , P < 0.05 ).

Following standard therapies, the percentages of circulating CD4+CXCR5+, CD4+CXCR5+PD-1+, and CD4+CXCR5+ICOS+ TFH cells and the levels of serum IL-21 were significantly reduced, but the levels of serum IL-4 and IL-10 were increased ( P < 0.05 ).

Conclusions.

A higher frequency of CD4+CXCR5+ TFH cells that existed in adult patients with MCD could be new target for intervention of MCD.