Fluvastatin Upregulates the α 1C Subunit of CaV1.2 Channel Expression in Vascular Smooth Muscle Cells via RhoA and ERKp38 MAPK Pathways
المؤلفون المشاركون
Ouyang, Qiufang
Han, Ying
Lin, Zhi-Hong
Xie, Hong
Xu, Chang-Sheng
Xie, Liang-Di
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-12-29
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Abnormal phenotypic switch of vascular smooth muscle cell (VSMC) is a hallmark of vascular disorders such as atherosclerosis and restenosis.
And this process has been related to remodeling of L-type calcium channel (LTCC).
We attempted to investigate whether fluvastatin has any effect on VSMC proliferation and LTCC α 1 C subunit ( L T C C α 1 C ) expression as well as the potential mechanisms involved.
The VSMCs proliferation was assayed by osteopontin immunofluorescent staining and [3H]-thymidine incorporation.
The cell cycle was detected by flow cytometric analysis.
The activity of RhoA was determined with pull-down assay.
MAPK activity and L T C C α 1 C expression were assessed by western blotting.
We demonstrated fluvastatin prevented the VSMCs dedifferentiating into a proliferative phenotype and induced cell cycle arrest in the G0/G1 phase in response to PDGF-BB stimulation.
Fluvastatin dose-dependently reversed the downregulation of L T C C α 1 C expression induced by PDGF-BB.
Inhibition of ROCK, ERK, or p38 MAPK activation largely enhanced the upregulation effect of fluvastatin ( P < 0.01 ) .
However, blockade of JNK pathway had no effect on L T C C α 1 C expression.
We concluded L T C C α 1 C was a VSMC contractile phenotype marker gene.
Fluvastatin upregulated L T C C α 1 C expression, at least in part, by inhibiting ROCK, ERK1/2, and p38 MAPK activation.
Fluvastatin may be a potential candidate for preventing or treating vascular diseases.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ouyang, Qiufang& Han, Ying& Lin, Zhi-Hong& Xie, Hong& Xu, Chang-Sheng& Xie, Liang-Di. 2014. Fluvastatin Upregulates the α 1C Subunit of CaV1.2 Channel Expression in Vascular Smooth Muscle Cells via RhoA and ERKp38 MAPK Pathways. Disease Markers،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1035051
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ouyang, Qiufang…[et al.]. Fluvastatin Upregulates the α 1C Subunit of CaV1.2 Channel Expression in Vascular Smooth Muscle Cells via RhoA and ERKp38 MAPK Pathways. Disease Markers No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1035051
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ouyang, Qiufang& Han, Ying& Lin, Zhi-Hong& Xie, Hong& Xu, Chang-Sheng& Xie, Liang-Di. Fluvastatin Upregulates the α 1C Subunit of CaV1.2 Channel Expression in Vascular Smooth Muscle Cells via RhoA and ERKp38 MAPK Pathways. Disease Markers. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1035051
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1035051
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر