Apoptosis Induction by the Total Flavonoids from Arachniodes exilis in HepG2 Cells through Reactive Oxygen Species-Mediated Mitochondrial Dysfunction Involving MAPK Activation
المؤلفون المشاركون
Li, Huimin
Chen, Jing
Xiong, Chaomei
Wei, Han
Yin, Changchang
Ruan, Jinlan
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-05-28
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Arachniodes exilis is used as a folk medicine in China and proved to have antibacterial, anti-inflammatory, and sedative activities.
In the present study, the antitumor effect of the total flavonoids of A.
exilis (TFAE) against HepG2 cells was evaluated.
The results showed that TFAE inhibited the growth of HepG2 cells in a dosage- and time-dependent manner.
Flow cytometry and Hoechst 33342 fluorescence staining results showed that TFAE could significantly increase the apoptosis ratio of HepG2 cells, which is accompanied with increased intracellular reactive oxygen species (ROS) production and decreased mitochondrial membrane potential (ΔΨm).
Western blotting indicated that TFAE downregulated the ratio of Bcl-2/Bax, increased cytochrome c release, and activated the caspases-3 and -9.
Further analysis showed that TFAE stimulated the mitogen-activated protein kinase (MAPK).
However, treatment with NAC (reactive oxygen species scavenger) and MAPK-specific inhibitors (SP600125 and SB203580) could reverse the changes of these apoptotic-related proteins.
These results suggested that TFAE possessed potential anticancer activity in HepG2 cells through ROS-mediated mitochondrial dysfunction involving MAPK pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Huimin& Chen, Jing& Xiong, Chaomei& Wei, Han& Yin, Changchang& Ruan, Jinlan. 2014. Apoptosis Induction by the Total Flavonoids from Arachniodes exilis in HepG2 Cells through Reactive Oxygen Species-Mediated Mitochondrial Dysfunction Involving MAPK Activation. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1035446
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Huimin…[et al.]. Apoptosis Induction by the Total Flavonoids from Arachniodes exilis in HepG2 Cells through Reactive Oxygen Species-Mediated Mitochondrial Dysfunction Involving MAPK Activation. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1035446
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Huimin& Chen, Jing& Xiong, Chaomei& Wei, Han& Yin, Changchang& Ruan, Jinlan. Apoptosis Induction by the Total Flavonoids from Arachniodes exilis in HepG2 Cells through Reactive Oxygen Species-Mediated Mitochondrial Dysfunction Involving MAPK Activation. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1035446
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1035446
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر