Bioactive Paper Sensor Based on the Acetylcholinesterase for the Rapid Detection of Organophosphate and Carbamate Pesticides
المؤلفون المشاركون
Badawy, Mohamed E. I.
El-Aswad, Ahmed F.
المصدر
International Journal of Analytical Chemistry
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-11-17
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
In many countries, people are becoming more concerned about pesticide residues which are present in or on food and feed products.
For this reason, several methods have been developed to monitor the pesticide residue levels in food samples.
In this study, a bioactive paper-based sensor was developed for detection of acetylcholinesterase (AChE) inhibitors including organophosphate and carbamate pesticides.
Based on the Ellman colorimetric assay, the assay strip is composed of a paper support ( 1 × 10 cm), onto which a biopolymer chitosan gel immobilized in crosslinking by glutaraldehyde with AChE and 5,5′-dithiobis(2-nitrobenzoic) acid (DTNB) and uses acetylthiocholine iodide (ATChI) as an outside reagent.
The assay protocol involves introducing the sample to sensing zone via dipping of a pesticide-containing solution.
Following an incubation period, the paper is placed into ATChI solution to initiate enzyme catalyzed hydrolysis of the substrate, causing a yellow color change.
The absence or decrease of the yellow color indicates the levels of the AChE inhibitors.
The biosensor is able to detect organophosphate and carbamate pesticides with good detection limits ( methomyl = 6.16 × 10 - 4 mM and profenofos = 0.27 mM) and rapid response times (~5 min).
The results show that the paper-based biosensor is rapid, sensitive, inexpensive, portable, disposable, and easy-to-use.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Badawy, Mohamed E. I.& El-Aswad, Ahmed F.. 2014. Bioactive Paper Sensor Based on the Acetylcholinesterase for the Rapid Detection of Organophosphate and Carbamate Pesticides. International Journal of Analytical Chemistry،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1035933
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Badawy, Mohamed E. I.& El-Aswad, Ahmed F.. Bioactive Paper Sensor Based on the Acetylcholinesterase for the Rapid Detection of Organophosphate and Carbamate Pesticides. International Journal of Analytical Chemistry No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1035933
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Badawy, Mohamed E. I.& El-Aswad, Ahmed F.. Bioactive Paper Sensor Based on the Acetylcholinesterase for the Rapid Detection of Organophosphate and Carbamate Pesticides. International Journal of Analytical Chemistry. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1035933
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1035933
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر