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Alpha Lipoic Acid Modulated High Glucose-Induced Rat Mesangial Cell Dysfunction via mTORp70S6K4E-BP1 Pathway
المؤلفون المشاركون
Lv, Chuan
Wu, Can
Zhou, Yue-hong
Shao, Ying
Wang, Guan
Wang, Qiu-yue
المصدر
International Journal of Endocrinology
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-10-29
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
The aim of this study was to investigate whether alpha lipoic acid (LA) regulates high glucose-induced mesangial cell proliferation and extracellular matrix production via mTOR/p70S6K/4E-BP1 signaling.
The effect of LA on high glucose-induced cell proliferation, fibronectin (FN), and collagen type I (collagen-I) expression and its mechanisms were examined in cultured rat mesangial cells by methylthiazol tetrazolium (MTT) assay, flow cytometry, ELISA assay, and western blot, respectively.
LA at a relatively low concentration (0.25 mmol/L) acted as a growth factor in rat mesangial cells, promoted entry of cell cycle into S phase, extracellular matrix formation, and phosphorylated AKT, mTOR, p70S6K, and 4E-BP1.
These effects disappeared when AKT expression was downregulated with PI3K/AKT inhibitor LY294002.
Conversely, LA at a higher concentration (1.0 mmol/L) inhibited high glucose-induced rat mesangial cell proliferation, entry of cell cycle into S phase, and extracellular matrix exertion, as well as phosphorylation of mTOR, p70S6K, and 4E-BP1 but enhanced the activity of AMPK.
However, these effects disappeared when AMPK activity was inhibited with CaMKK inhibitor STO-609.
These results suggest that LA dose-dependently regulates mesangial cell proliferation and matrix protein secretion by mTOR/p70S6K/4E-BP1 signaling pathway under high glucose conditions.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lv, Chuan& Wu, Can& Zhou, Yue-hong& Shao, Ying& Wang, Guan& Wang, Qiu-yue. 2014. Alpha Lipoic Acid Modulated High Glucose-Induced Rat Mesangial Cell Dysfunction via mTORp70S6K4E-BP1 Pathway. International Journal of Endocrinology،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-1036554
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lv, Chuan…[et al.]. Alpha Lipoic Acid Modulated High Glucose-Induced Rat Mesangial Cell Dysfunction via mTORp70S6K4E-BP1 Pathway. International Journal of Endocrinology No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-1036554
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lv, Chuan& Wu, Can& Zhou, Yue-hong& Shao, Ying& Wang, Guan& Wang, Qiu-yue. Alpha Lipoic Acid Modulated High Glucose-Induced Rat Mesangial Cell Dysfunction via mTORp70S6K4E-BP1 Pathway. International Journal of Endocrinology. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-1036554
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1036554
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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