Transcriptome of the Deep-Sea Black Scabbardfish, Aphanopus carbo (Perciformes: Trichiuridae): Tissue-Specific Expression Patterns and Candidate Genes Associated to Depth Adaptation
المؤلفون المشاركون
Stefanni, Sergio
Bettencourt, Raul
Pinheiro, Miguel
Moro, Gianluca De
Bongiorni, Lucia
Pallavicini, Alberto
المصدر
International Journal of Genomics
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-21، 21ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-09-17
دولة النشر
مصر
عدد الصفحات
21
التخصصات الرئيسية
الملخص EN
Deep-sea fishes provide a unique opportunity to study the physiology and evolutionary adaptation to extreme environments.
We carried out a high throughput sequencing analysis on a 454 GS-FLX titanium plate using unnormalized cDNA libraries from six tissues of A.
carbo.
Assemblage and annotations were performed by Newbler and InterPro/Pfam analyses, respectively.
The assembly of 544,491 high quality reads provided 8,319 contigs, 55.6% of which retrieved blast hits against the NCBI nonredundant database or were annotated with ESTscan.
Comparison of functional genes at both the protein sequences and protein stability levels, associated with adaptations to depth, revealed similarities between A.
carbo and other bathypelagic fishes.
A selection of putative genes was standardized to evaluate the correlation between number of contigs and their normalized expression, as determined by qPCR amplification.
The screening of the libraries contributed to the identification of new EST simple-sequence repeats (SSRs) and to the design of primer pairs suitable for population genetic studies as well as for tagging and mapping of genes.
The characterization of the deep-sea fish A.
carbo first transcriptome is expected to provide abundant resources for genetic, evolutionary, and ecological studies of this species and the basis for further investigation of depth-related adaptation processes in fishes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Stefanni, Sergio& Bettencourt, Raul& Pinheiro, Miguel& Moro, Gianluca De& Bongiorni, Lucia& Pallavicini, Alberto. 2014. Transcriptome of the Deep-Sea Black Scabbardfish, Aphanopus carbo (Perciformes: Trichiuridae): Tissue-Specific Expression Patterns and Candidate Genes Associated to Depth Adaptation. International Journal of Genomics،Vol. 2014, no. 2014, pp.1-21.
https://search.emarefa.net/detail/BIM-1036687
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Stefanni, Sergio…[et al.]. Transcriptome of the Deep-Sea Black Scabbardfish, Aphanopus carbo (Perciformes: Trichiuridae): Tissue-Specific Expression Patterns and Candidate Genes Associated to Depth Adaptation. International Journal of Genomics No. 2014 (2014), pp.1-21.
https://search.emarefa.net/detail/BIM-1036687
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Stefanni, Sergio& Bettencourt, Raul& Pinheiro, Miguel& Moro, Gianluca De& Bongiorni, Lucia& Pallavicini, Alberto. Transcriptome of the Deep-Sea Black Scabbardfish, Aphanopus carbo (Perciformes: Trichiuridae): Tissue-Specific Expression Patterns and Candidate Genes Associated to Depth Adaptation. International Journal of Genomics. 2014. Vol. 2014, no. 2014, pp.1-21.
https://search.emarefa.net/detail/BIM-1036687
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1036687
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر