![](/images/graphics-bg.png)
Identification and Characterization of Microsatellites in Expressed Sequence Tags and Their Cross Transferability in Different Plants
المؤلفون المشاركون
Haq, Shamshad ul
Jain, Rohit
Sharma, Meenakshi
Kachhwaha, Sumita
Kothari, S. L.
المصدر
International Journal of Genomics
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-10-19
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Expressed sequence tags (EST) are potential source for the development of genic microsatellite markers, gene discovery, comparative genomics, and other genomic studies.
In the present study, 7630 ESTs were examined from NCBI for SSR identification and characterization.
A total of 263 SSRs were identified with an average density of one SSR/4.2 kb (3.4% frequency).
Analysis revealed that trinucleotide repeats (47.52%) were most abundant followed by tetranucleotide (19.77%), dinucleotide (19.01%), pentanucleotide (9.12%), and hexanucleotide repeats (4.56%).
Functional annotation was done through homology search and gene ontology, and 35 EST-SSRs were selected.
Primer pairs were designed for evaluation of cross transferability and polymorphism among 11 plants belonging to five different families.
Total 402 alleles were generated at 155 loci with an average of 2.6 alleles/locus and the polymorphic information content (PIC) ranged from 0.15 to 0.92 with an average of 0.75.
The cross transferability ranged from 34.84% to 98.06% in different plants, with an average of 67.86%.
Thus, the validation study of annotated 35 EST-SSR markers which correspond to particular metabolic activity revealed polymorphism and evolutionary nature in different families of Angiospermic plants.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Haq, Shamshad ul& Jain, Rohit& Sharma, Meenakshi& Kachhwaha, Sumita& Kothari, S. L.. 2014. Identification and Characterization of Microsatellites in Expressed Sequence Tags and Their Cross Transferability in Different Plants. International Journal of Genomics،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1036696
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Haq, Shamshad ul…[et al.]. Identification and Characterization of Microsatellites in Expressed Sequence Tags and Their Cross Transferability in Different Plants. International Journal of Genomics No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1036696
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Haq, Shamshad ul& Jain, Rohit& Sharma, Meenakshi& Kachhwaha, Sumita& Kothari, S. L.. Identification and Characterization of Microsatellites in Expressed Sequence Tags and Their Cross Transferability in Different Plants. International Journal of Genomics. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1036696
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1036696
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)