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Development of a Univariate Membrane-Based Mid-Infrared Method for Protein Quantitation and Total Lipid Content Analysis of Biological Samples
المؤلفون المشاركون
Strug, Ivona
Utzat, Christopher
Cappione, Amedeo
Gutierrez, Sara
Amara, Ryan
Lento, Joseph
Capito, Florian
Skudas, Romas
Chernokalskaya, Elena
Nadler, Timothy
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-10-13
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Biological samples present a range of complexities from homogeneous purified protein to multicomponent mixtures.
Accurate qualification of such samples is paramount to downstream applications.
We describe the development of an MIR spectroscopy-based analytical method offering simultaneous protein quantitation (0.25–5 mg/mL) and analysis of total lipid or detergent species, as well as the identification of other biomolecules present in biological samples.
The method utilizes a hydrophilic PTFE membrane engineered for presentation of aqueous samples in a dried format compatible with fast infrared analysis.
Unlike classical quantification techniques, the reported method is amino acid sequence independent and thus applicable to complex samples of unknown composition.
By comparison to existing platforms, this MIR-based method enables direct quantification using minimal sample volume (2 µL); it is well-suited where repeat access and limited sample size are critical parameters.
Further, accurate results can be derived without specialized training or knowledge of IR spectroscopy.
Overall, the simplified application and analysis system provides a more cost-effective alternative to high-throughput IR systems for research laboratories with minimal throughput demands.
In summary, the MIR-based system provides a viable alternative to current protein quantitation methods; it also uniquely offers simultaneous qualification of other components, notably lipids and detergents.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Strug, Ivona& Utzat, Christopher& Cappione, Amedeo& Gutierrez, Sara& Amara, Ryan& Lento, Joseph…[et al.]. 2014. Development of a Univariate Membrane-Based Mid-Infrared Method for Protein Quantitation and Total Lipid Content Analysis of Biological Samples. Journal of Analytical Methods in Chemistry،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1039836
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Strug, Ivona…[et al.]. Development of a Univariate Membrane-Based Mid-Infrared Method for Protein Quantitation and Total Lipid Content Analysis of Biological Samples. Journal of Analytical Methods in Chemistry No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1039836
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Strug, Ivona& Utzat, Christopher& Cappione, Amedeo& Gutierrez, Sara& Amara, Ryan& Lento, Joseph…[et al.]. Development of a Univariate Membrane-Based Mid-Infrared Method for Protein Quantitation and Total Lipid Content Analysis of Biological Samples. Journal of Analytical Methods in Chemistry. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1039836
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1039836
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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