Hyperglycemia Induces Toll-Like Receptor-2 and -4 Expression and Activity in Human Microvascular Retinal Endothelial Cells: Implications for Diabetic Retinopathy
المؤلفون المشاركون
Jialal, Ishwarlal
Rajamani, Uthra
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-12-30
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Diabetic retinopathy (DR) causes visual impairment in working age adults and hyperglycemia-mediated inflammation is central in DR.
Toll-like receptors (TLRs) play a key role in innate immune responses and inflammation.
However, scanty data is available on their role in DR.
Hence, in this study, we examined TLR2 and TLR4 mRNA and protein expression and activity in hyperglycemic human retinal endothelial cells (HMVRECs).
HMVRECs were treated with hyperglycemia (HG) or euglycemia and mRNA and protein levels of TLR-2, TLR-4, MyD88, IRF3, and TRIF as well as NF-κB p65 activation were measured.
IL-8, IL-1β, TNF-α and MCP-1, ICAM-1, and VCAM-1 as well as monocyte adhesion to HMVRECs were also assayed.
HG (25 mM) significantly induced TLR2 and TLR4 mRNA and protein in HMVRECs.
It also increased both MyD88 and non-MyD88 pathways, nuclear factor-κB (NF-κB), biomediators, and monocyte adhesion.
This inflammation was attenuated by TLR-4 or TLR-2 inhibition, and dual inhibition by a TLR inhibitory peptide as well as TLR2 and 4 siRNA.
Additionally, antioxidant treatment reduced TLR-2 and TLR4 expression and downstream inflammatory markers.
Collectively, our novel data suggest that hyperglycemia induces TLR-2 and TLR-4 activation and downstream signaling mediating increased inflammation possibly via reactive oxygen species (ROS) and could contribute to DR.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Rajamani, Uthra& Jialal, Ishwarlal. 2014. Hyperglycemia Induces Toll-Like Receptor-2 and -4 Expression and Activity in Human Microvascular Retinal Endothelial Cells: Implications for Diabetic Retinopathy. Journal of Diabetes Research،Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1040371
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Rajamani, Uthra& Jialal, Ishwarlal. Hyperglycemia Induces Toll-Like Receptor-2 and -4 Expression and Activity in Human Microvascular Retinal Endothelial Cells: Implications for Diabetic Retinopathy. Journal of Diabetes Research No. 2014 (2014), pp.1-15.
https://search.emarefa.net/detail/BIM-1040371
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Rajamani, Uthra& Jialal, Ishwarlal. Hyperglycemia Induces Toll-Like Receptor-2 and -4 Expression and Activity in Human Microvascular Retinal Endothelial Cells: Implications for Diabetic Retinopathy. Journal of Diabetes Research. 2014. Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1040371
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1040371
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر