Optimization of Unnicked β2-Glycoprotein I and High Avidity Anti- β2-Glycoprotein I Antibodies Isolation
المؤلفون المشاركون
Ambrožič, Aleš
Artenjak, Andrej
Leonardi, Adrijana
Križaj, Igor
Božič, Borut
Čučnik, Saša
Sodin-Semrl, Snezna
المصدر
Journal of Immunology Research
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-01-23
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Patient biological material for isolation of β2-glycoprotein I (β2GPI) and high avidity IgG anti-β2-glycoprotein I antibodies (HAv anti-β2GPI) dictates its full utilization.
The aim of our study was to evaluate/improve procedures for isolation of unnicked β2GPI and HAv aβ2GPI to gain unmodified proteins in higher yields/purity.
Isolation of β2GPI from plasma was a stepwise procedure combining nonspecific and specific methods.
For isolation of polyclonal HAv aβ2GPI affinity chromatographies with immobilized protein G and human β2GPI were used.
The unknown protein found during isolation was identified by liquid chromatography electrospray ionization mass spectrometry and the nonredundant National Center for Biotechnology Information database.
The average mass of the isolated unnicked purified β2GPI increased from 6.56 mg to 9.94 mg.
In the optimized isolation procedure the high molecular weight protein (proteoglycan 4) was successfully separated from β2GPI in the 1st peaks with size exclusion chromatography.
The average efficiency of the isolation procedure for polyclonal HAv anti-β2GPI from different matrixes was 13.8%, as determined by our in-house anti-β2GPI ELISA.
We modified the in-house isolation and purification procedures of unnicked β2GPI and HAv anti-β2GPI, improving the purity of antigen and antibodies as well as increasing the number of tests routinely performed with the in-house ELISA by ~50%.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Artenjak, Andrej& Leonardi, Adrijana& Križaj, Igor& Ambrožič, Aleš& Sodin-Semrl, Snezna& Božič, Borut…[et al.]. 2014. Optimization of Unnicked β2-Glycoprotein I and High Avidity Anti- β2-Glycoprotein I Antibodies Isolation. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1040787
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Artenjak, Andrej…[et al.]. Optimization of Unnicked β2-Glycoprotein I and High Avidity Anti- β2-Glycoprotein I Antibodies Isolation. Journal of Immunology Research No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1040787
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Artenjak, Andrej& Leonardi, Adrijana& Križaj, Igor& Ambrožič, Aleš& Sodin-Semrl, Snezna& Božič, Borut…[et al.]. Optimization of Unnicked β2-Glycoprotein I and High Avidity Anti- β2-Glycoprotein I Antibodies Isolation. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1040787
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1040787
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر