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Construction of a Chimeric Secretory IgA and Its Neutralization Activity against Avian Influenza Virus H5N1
المؤلفون المشاركون
Tong, Yigang
Huang, Yong
Li, Cun
An, Xiaoping
Butt, Azeem Mehmood
Zhang, Baozhong
Zhang, Zhiyi
Wang, Xiaona
Zhang, Wenhui
Zhang, Bo
Mi, Zhiqiang
المصدر
Journal of Immunology Research
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-02-13
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Secretory immunoglobulin A (SIgA) acts as the first line of defense against respiratory pathogens.
In this assay, the variable regions of heavy chain (VH) and Light chain (VL) genes from a mouse monoclonal antibody against H5N1 were cloned and fused with human IgA constant regions.
The full-length chimeric light and heavy chains were inserted into a eukaryotic expressing vector and then transfected into CHO/dhfr-cells.
The chimeric monomeric IgA antibody expression was confirmed by using ELISA, SDS-PAGE, and Western blot.
In order to obtain a dimeric secretory IgA, another two expressing plasmids, namely, pcDNA4/His A-IgJ and pcDNA4/His A-SC, were cotransfected into the CHO/dhfr-cells.
The expression of dimeric SIgA was confirmed by using ELISA assay and native gel electrophoresis.
In microneutralization assay on 96-well immunoplate, the chimeric SIgA showed neutralization activity against H5N1 virus on MDCK cells and the titer was determined to be 1 : 64.
On preadministrating intranasally, the chimeric SIgA could prevent mice from lethal attack by using A/Vietnam/1194/04 H5N1 with a survival rate of 80%.
So we concluded that the constructed recombinant chimeric SIgA has a neutralization capability targeting avian influenza virus H5N1 infection in vitro and in vivo.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Cun& An, Xiaoping& Butt, Azeem Mehmood& Zhang, Baozhong& Zhang, Zhiyi& Wang, Xiaona…[et al.]. 2014. Construction of a Chimeric Secretory IgA and Its Neutralization Activity against Avian Influenza Virus H5N1. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1040859
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Cun…[et al.]. Construction of a Chimeric Secretory IgA and Its Neutralization Activity against Avian Influenza Virus H5N1. Journal of Immunology Research No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1040859
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Cun& An, Xiaoping& Butt, Azeem Mehmood& Zhang, Baozhong& Zhang, Zhiyi& Wang, Xiaona…[et al.]. Construction of a Chimeric Secretory IgA and Its Neutralization Activity against Avian Influenza Virus H5N1. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1040859
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1040859
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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