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Flow Cytometric Detection of p38 MAPK Phosphorylation and Intracellular Cytokine Expression in Peripheral Blood Subpopulations from Patients with Autoimmune Rheumatic Diseases
المؤلفون المشاركون
Sakkas, Lazaros I.
Bogdanos, Dimitrios P.
Simopoulou, Theodora
Zafiriou, Efterpi
Liaskos, Christos
Mavropoulos, Athanasios
Orfanidou, Timoklia
Rigopoulou, Eirini I.
المصدر
Journal of Immunology Research
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-02-03
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Flow cytometric analysis of p38 mitogen-activated protein kinase (p38 MAPK) signaling cascade is optimally achieved by methanol permeabilization protocols.
Such protocols suffer from the difficulties to accurately detect intracellular cytokines and surface epitopes of infrequent cell subpopulations, which are removed by methanol.
To overcome these limitations, we have modified methanol-based phosphoflow protocols using several commercially available antibody clones suitable for surface antigens, intracellular cytokines, and p38 MAPK.
These included markers of B cells (CD19, CD20, and CD22), T cells (CD3, CD4, and CD8), NK (CD56 and CD7), and dendritic cells (CD11c).
We have also tested surface markers of costimulatory molecules, such as CD27.
We have successfully determined simultaneous expression of IFN-γ, as well as IL-10, and phosphorylated p38 in cell subsets.
The optimized phosphoflow protocol has also been successfully applied in peripheral blood mononuclear cells or purified cell subpopulations from patients with various autoimmune diseases.
In conclusion, our refined phosphoflow cytometric approach allows simultaneous detection of p38 MAPK activity and intracellular cytokine expression and could be used as an important tool to study signaling cascades in autoimmunity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mavropoulos, Athanasios& Bogdanos, Dimitrios P.& Liaskos, Christos& Orfanidou, Timoklia& Simopoulou, Theodora& Zafiriou, Efterpi…[et al.]. 2014. Flow Cytometric Detection of p38 MAPK Phosphorylation and Intracellular Cytokine Expression in Peripheral Blood Subpopulations from Patients with Autoimmune Rheumatic Diseases. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1040942
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mavropoulos, Athanasios…[et al.]. Flow Cytometric Detection of p38 MAPK Phosphorylation and Intracellular Cytokine Expression in Peripheral Blood Subpopulations from Patients with Autoimmune Rheumatic Diseases. Journal of Immunology Research No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-1040942
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mavropoulos, Athanasios& Bogdanos, Dimitrios P.& Liaskos, Christos& Orfanidou, Timoklia& Simopoulou, Theodora& Zafiriou, Efterpi…[et al.]. Flow Cytometric Detection of p38 MAPK Phosphorylation and Intracellular Cytokine Expression in Peripheral Blood Subpopulations from Patients with Autoimmune Rheumatic Diseases. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1040942
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1040942
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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