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Conventional Rapid Latex Agglutination in Estimation of von Willebrand Factor: Method Revisited and Potential Clinical Applications
المؤلفون المشاركون
Mahat, Marianor
Abdullah, Wan Zaidah
Che Hussin, Che Maraina
المصدر
Journal of Immunology Research
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-12-25
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Measurement of von Willebrand factor antigen (VWF : Ag) levels is usually performed in a specialised laboratory which limits its application in routine clinical practice.
So far, no commercial rapid test kit is available for VWF : Ag estimation.
This paper discusses the technical aspect of latex agglutination method which was established to suit the purpose of estimating von Willebrand factor (VWF) levels in the plasma sample.
The latex agglutination test can be performed qualitatively and semiquantitatively.
Reproducibility, stability, linearity, limit of detection, interference, and method comparison studies were conducted to evaluate the performance of this test.
Semiquantitative latex agglutination test was strongly correlated with the reference immunoturbidimetric assay (Spearman’s rho = 0.946, P<0.001, n=132).
A substantial agreement (κ=0.77) was found between qualitative latex agglutination test and the reference assay.
Using the scoring system for the rapid latex test, no agglutination is with 0% VWF : Ag (control negative), 1+ reaction is equivalent to <20% VWF : Ag, and 4+ reaction indicates >150% VWF : Ag (when comparing with immunoturbidimetric assay).
The findings from evaluation studies suggest that latex agglutination method is suitable to be used as a rapid test kit for the estimation of VWF : Ag levels in various clinical conditions associated with high levels and low levels of VWF : Ag.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mahat, Marianor& Abdullah, Wan Zaidah& Che Hussin, Che Maraina. 2014. Conventional Rapid Latex Agglutination in Estimation of von Willebrand Factor: Method Revisited and Potential Clinical Applications. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1040999
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mahat, Marianor…[et al.]. Conventional Rapid Latex Agglutination in Estimation of von Willebrand Factor: Method Revisited and Potential Clinical Applications. Journal of Immunology Research No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1040999
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mahat, Marianor& Abdullah, Wan Zaidah& Che Hussin, Che Maraina. Conventional Rapid Latex Agglutination in Estimation of von Willebrand Factor: Method Revisited and Potential Clinical Applications. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1040999
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1040999
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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