Incubation of MDCO-216 (ApoA-IMilanoPOPC) with Human Serum Potentiates ABCA1-Mediated Cholesterol Efflux Capacity, Generates New Prebeta-1 HDL, and Causes an Increase in HDL Size
المؤلفون المشاركون
Asztalos, Bela F.
Kempen, Herman J.
Schranz, Dorota B.
Otvos, James
Jeyarajah, Elias
Drazul-Schrader, Denise
Collins, Heidi L.
Adelman, Steven J.
Wijngaard, Peter L. J.
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-11-12
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
MDCO-216 is a complex of dimeric ApoA-IMilano and palmitoyl oleoyl phosphatidylcholine (POPC), previously shown to reduce atherosclerotic plaque burden.
Here we studied the effect of incubation of human plasma or serum with MDCO-216 on cholesterol efflux capacity from J774 cells, on prebeta-1 high density lipoprotein (prebeta-1 HDL) and on HDL size assessed by proton nuclear magnetic resonance (1H-NMR).
MDCO-216 incubated in buffer containing 4% human serum albumin stimulated both ABCA1-mediated efflux and ABCA1-independent cholesterol efflux from J774 macrophages.
When incubated with human serum a dose- and time-dependent synergistic increase of the ABCA1-mediated efflux capacity were observed.
Using a commercially available ELISA for prebeta-1 HDL, MDCO-216 as such was poorly detected (12–15% of nominal amount of protein).
Prebeta-1 HDL was rapidly lost when human plasma alone is incubated at 37°C.
In contrast, incubation of human plasma with MDCO-216 at 37°C produced a large amount of new prebeta-1 HDL.
Native 2D electrophoresis followed by immunoblotting with an apoA-I antibody, which also detects ApoA-I Milano, confirmed the increase in prebeta-1 HDL upon incubation at 37°C.
With the increase of prebeta-1 HDL, the concomitant disappearance of the small alpha-3 and alpha-4 HDL and MDCO-216 and an increase in the large alpha-1 and alpha-2 HDL were observed.
Immunoblotting with Mab 17F3 specific for ApoA-I Milano showed the appearance of ApoA-I Milano in alpha-1 and alpha-2, but not in prebeta-1 HDL.
1H-NMR analysis of plasma incubated with MDCO-216 confirmed rapid disappearance of small-sized HDL particles and increase of medium- and large-sized HDL particles accompanied with a decrease in total HDL particle number.
In conclusion, incubation of human plasma or serum with MDCO-216 strongly enhanced ABCA1-mediated cholesterol efflux, caused a strong increase of prebeta-1 HDL, and drastically changed the distribution of HDL subpopulations.
Overall, the results are in line with the hypothesis that MDCO-216 fuses with small alpha-migrating HDL particles forming larger particles containing both apoA-I WT and ApoA-I Milano, meanwhile liberating the endogenous wild-type apoA-I which enriches prebeta-1 HDL subpopulation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kempen, Herman J.& Schranz, Dorota B.& Asztalos, Bela F.& Otvos, James& Jeyarajah, Elias& Drazul-Schrader, Denise…[et al.]. 2014. Incubation of MDCO-216 (ApoA-IMilanoPOPC) with Human Serum Potentiates ABCA1-Mediated Cholesterol Efflux Capacity, Generates New Prebeta-1 HDL, and Causes an Increase in HDL Size. Journal of Lipids،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1041060
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kempen, Herman J.…[et al.]. Incubation of MDCO-216 (ApoA-IMilanoPOPC) with Human Serum Potentiates ABCA1-Mediated Cholesterol Efflux Capacity, Generates New Prebeta-1 HDL, and Causes an Increase in HDL Size. Journal of Lipids No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1041060
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kempen, Herman J.& Schranz, Dorota B.& Asztalos, Bela F.& Otvos, James& Jeyarajah, Elias& Drazul-Schrader, Denise…[et al.]. Incubation of MDCO-216 (ApoA-IMilanoPOPC) with Human Serum Potentiates ABCA1-Mediated Cholesterol Efflux Capacity, Generates New Prebeta-1 HDL, and Causes an Increase in HDL Size. Journal of Lipids. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1041060
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1041060
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر