Reliability of a Fully Automated Interpretation of γ-H2AX Foci in Lymphocytes of Moderately Trained Subjects under Resting Conditions
المؤلفون المشاركون
Heydenreich, Juliane
Otto, Christoph
Mayer, Frank
Carlsohn, Anja
المصدر
Journal of Nutrition and Metabolism
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-07-23
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Background.
Analysis of γ-H2AX foci is a promising approach to evaluate exercise-induced DNA damage.
However, baseline levels and day-to-day variability of γ-H2AX foci have not been investigated in healthy subjects at rest.
Methods.
Blood was taken from eight moderately trained healthy males (29 ± 3 yrs, 1.84 ± 0.03 m, and 85 ± 6 kg) at two separate days (M1/M2) after 24-hour exercise cessation.
Number of γ-H2AX foci per 100 lymphocytes (N), number of foci per affected lymphocyte (NAL), percentage of affected lymphocytes (PAL), and diameter (D) of γ-H2AX foci were analyzed (mean ± SD).
Differences between M1 and M2 were analyzed using paired t-tests (α = 0.05).
Day-to-day variability was evaluated by calculating the coefficients of variation (CV%), bias, and limits of agreement (LoA).
Results.
There were no statistically significant differences between M1 (N: 7.6 ± 4.4, NAL: 1.2 ± 0.2, PAL: 5.9 ± 2.6%, and D: 0.63 ± 0.07) and M2 (N: 8.4 ± 4.6, NAL: 1.3 ± 0.1, PAL: 6.9 ± 4.2%, and D: 0.66 ± 0.06).
CV was calculated to be 98.5% (N), 88.9% (PAL), 11.3% (NAL), and 8.0% (D).
Bias (LoA) was 0.75 (−15.2/13.7), −0.02 (−0.36/0.33), −1.0 (−11.9/9.9), and −0.04 (−0.16/0.09), respectively.
Conclusions.
Background level in healthy subjects is approximately 0.07 to 0.09 γ-H2AX foci/cell.
NAL and D are reliable measures.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Heydenreich, Juliane& Otto, Christoph& Mayer, Frank& Carlsohn, Anja. 2014. Reliability of a Fully Automated Interpretation of γ-H2AX Foci in Lymphocytes of Moderately Trained Subjects under Resting Conditions. Journal of Nutrition and Metabolism،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1042161
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Heydenreich, Juliane…[et al.]. Reliability of a Fully Automated Interpretation of γ-H2AX Foci in Lymphocytes of Moderately Trained Subjects under Resting Conditions. Journal of Nutrition and Metabolism No. 2014 (Dec. 2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1042161
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Heydenreich, Juliane& Otto, Christoph& Mayer, Frank& Carlsohn, Anja. Reliability of a Fully Automated Interpretation of γ-H2AX Foci in Lymphocytes of Moderately Trained Subjects under Resting Conditions. Journal of Nutrition and Metabolism. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1042161
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1042161
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر