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Effective Concentration and Detection of Cryptosporidium, Giardia, and the Microsporidia from Environmental Matrices
المؤلفون المشاركون
Moss, Joseph A.
Gordy, John
Snyder, Richard A.
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-09-10
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Cryptosporidium spp., Giardia spp., and members of Microsporidia are enteropathogenic parasites of humans and animals, producing asymptomatic to severe intestinal infections.
To circumvent various impediments associated with current detection methods, we tested a method providing multistage purification and separation in a single, confined step.
Standard real-time PCR was used as a detection method.
Samples spiked with C.
parvum and G.
intestinalis were split for comparison to standard Method 1623.
Results were equivalent to immunomagnetic procedures for Cryptosporidium, and Giardia.
Overall percent recovery for Cryptosporidium with Method 1623 averaged 26.89% (std 21.44%; min = 0%; max = 73%) and was similar but less variable for qPCR method at an estimated average of 27.67 (std 17.65%; min = 5%; max = 63%).
For Giardia, Method 1623 had an overall average recovery of 27.11% (std 17.98%; min = 1%; max = 58%), while multistage purification and qPCR had an estimated lower overall recovery at 18.58% (std 13.95%; min = 0%; max = 35%).
Microsporidia were also readily detected with an estimated recovery of 46.81% overall (std 17.66%; min = 18%; max = 70%) for E.
intestinalis and 38.90% (std 14.36%; min = 13%; max = 62%) for E.
bieneusi.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Moss, Joseph A.& Gordy, John& Snyder, Richard A.. 2014. Effective Concentration and Detection of Cryptosporidium, Giardia, and the Microsporidia from Environmental Matrices. Journal of Pathogens،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1042769
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Moss, Joseph A.…[et al.]. Effective Concentration and Detection of Cryptosporidium, Giardia, and the Microsporidia from Environmental Matrices. Journal of Pathogens No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1042769
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Moss, Joseph A.& Gordy, John& Snyder, Richard A.. Effective Concentration and Detection of Cryptosporidium, Giardia, and the Microsporidia from Environmental Matrices. Journal of Pathogens. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1042769
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1042769
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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