![](/images/graphics-bg.png)
Diagnostic Utility of Broad Range Bacterial 16S rRNA Gene PCR with Degradation of Human and Free Bacterial DNA in Bloodstream Infection Is More Sensitive Than an In-House Developed PCR without Degradation of Human and Free Bacterial DNA
المؤلفون المشاركون
Rogina, Petra
Skvarc, Miha
Stubljar, David
Kofol, Romina
Kaasch, Achim
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-07-09
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
We compared a commercial broad range 16S rRNA gene PCR assay (SepsiTest) to an in-house developed assay (IHP).
We assessed whether CD64 index, a biomarker of bacterial infection, can be used to exclude patients with a low probability of systemic bacterial infection.
From January to March 2010, 23 patients with suspected sepsis were enrolled.
CD64 index, procalcitonin, and C-reactive protein were measured on admission.
Broad range 16S rRNA gene PCR was performed from whole blood (SepsiTest) or blood plasma (IHP) and compared to blood culture results.
Blood samples spiked with Staphylococcus aureus were used to assess sensitivity of the molecular assays in vitro.
CD64 index was lower in patients where possible sepsis was excluded than in patients with microbiologically confirmed sepsis ( P = 0.004 ).
SepsiTest identified more relevant pathogens than blood cultures ( P = 0.008 ); in three patients (13%) results from blood culture and SepsiTest were congruent, whereas in four cases (17.4%) relevant pathogens were detected by SepsiTest only.
In vitro spiking experiments suggested equal sensitivity of SepsiTest and IHP.
A diagnostic algorithm using CD64 index as a decision maker to perform SepsiTest shows improved detection of pathogens in patients with suspected blood stream infection and may enable earlier targeted antibiotic therapy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Rogina, Petra& Skvarc, Miha& Stubljar, David& Kofol, Romina& Kaasch, Achim. 2014. Diagnostic Utility of Broad Range Bacterial 16S rRNA Gene PCR with Degradation of Human and Free Bacterial DNA in Bloodstream Infection Is More Sensitive Than an In-House Developed PCR without Degradation of Human and Free Bacterial DNA. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1043312
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Rogina, Petra…[et al.]. Diagnostic Utility of Broad Range Bacterial 16S rRNA Gene PCR with Degradation of Human and Free Bacterial DNA in Bloodstream Infection Is More Sensitive Than an In-House Developed PCR without Degradation of Human and Free Bacterial DNA. Mediators of Inflammation No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1043312
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Rogina, Petra& Skvarc, Miha& Stubljar, David& Kofol, Romina& Kaasch, Achim. Diagnostic Utility of Broad Range Bacterial 16S rRNA Gene PCR with Degradation of Human and Free Bacterial DNA in Bloodstream Infection Is More Sensitive Than an In-House Developed PCR without Degradation of Human and Free Bacterial DNA. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1043312
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1043312
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)