Astrocyte Elevated Gene-1 Mediates Glycolysis and Tumorigenesis in Colorectal Carcinoma Cells via AMPK Signaling
المؤلفون المشاركون
Song, Hong-tao
Qin, Yu
Yao, Guo-dong
Tian, Zhen-nan
Fu, Song-bin
Geng, Jing-shu
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-04-16
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
To investigate the role of AEG-1 in glycolysis and tumorigenesis, we construct myc-AEG-1 expression vector and demonstrate a novel mechanism that AEG-1 may increase the activity of AMPK by Thr172 phosphorylation.
The higher expression levels of AEG-1 in colorectal carcinoma cells were found but showed significant difference in different cell lines.
To study the role of AEG-1 in colorectal cells, myc-AEG-1 vector was constructed and transfected into NCM460 colonic epithelial cells.
We observed consistent increasing of glucose consumption and lactate production, typical features of anaerobic glycolysis, suggesting that AEG-1 may promote anaerobic glycolysis.
Moreover, we noted that AMPK phosphorylation at Thr172 as well as pPFK2 (Ser466) was increased in NCM460 cells overexpressing AEG-1.
Compound C may block AMPK and PFK2 phosphorylation in both control and AEG-1-overexpressed cells and decrease the glucose consumption and lactate production.
The present findings indicated that reduced AEG-1 protein levels by RNAi may decrease the glucose consumption and lactate production in HCT116 colorectal carcinoma cells.
The present identified AEG-1/AMPK/PFK2 glycolysis cascade may be essential to cell proliferation and tumor growth.
The present results may provide us with a mechanistic insight into novel targets controlled by AEG-1, and the components in the AEG-1/AMPK/PFK2 glycolysis process may be targeted for the clinical treatment of cancer.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Song, Hong-tao& Qin, Yu& Yao, Guo-dong& Tian, Zhen-nan& Fu, Song-bin& Geng, Jing-shu. 2014. Astrocyte Elevated Gene-1 Mediates Glycolysis and Tumorigenesis in Colorectal Carcinoma Cells via AMPK Signaling. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1043420
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Song, Hong-tao…[et al.]. Astrocyte Elevated Gene-1 Mediates Glycolysis and Tumorigenesis in Colorectal Carcinoma Cells via AMPK Signaling. Mediators of Inflammation No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1043420
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Song, Hong-tao& Qin, Yu& Yao, Guo-dong& Tian, Zhen-nan& Fu, Song-bin& Geng, Jing-shu. Astrocyte Elevated Gene-1 Mediates Glycolysis and Tumorigenesis in Colorectal Carcinoma Cells via AMPK Signaling. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1043420
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1043420
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر