Porcine Reproductive and Respiratory Syndrome Virus Induces IL-1β Production Depending on TLR4MyD88 Pathway and NLRP3 Inflammasome in Primary Porcine Alveolar Macrophages

المؤلفون المشاركون

Xiao, Shaobo
Bi, Jing
Song, Shuang
Fang, Liurong
Wang, Dang
Jing, Huiyuan
Gao, Li
Cai, Yidong
Luo, Rui
Xiao, Shaobo

المصدر

Mediators of Inflammation

العدد

المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-14، 14ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2014-05-21

دولة النشر

مصر

عدد الصفحات

14

التخصصات الرئيسية

الأمراض

الملخص EN

Porcine reproductive and respiratory syndrome virus (PRRSV) is an Arterivirus that has been devastating the swine industry worldwide since the late 1980s.

Previous studies have reported that PRRSV infection induced the production of IL-1β.

However, the cellular sensors and signaling pathways involved in this process have not been elucidated yet.

Here, we studied the mechanisms responsible for the production of IL-1β in response to highly pathogenic PRRSV.

Upon PRRSV infection of primary porcine alveolar macrophages, both mRNA expression and secretion of IL-1β were significantly increased in a time- and dose-dependent manner.

We also investigated the role of several pattern-recognition receptors and adaptor molecules in this response and showed that the TLR4/MyD88 pathway and its downstream signaling molecules, NF-κB, ERK1/2, and p38 MAPKs, were involved in IL-1β production during PRRSV infection.

Treatment with specific inhibitors or siRNA knockdown assays demonstrated that components of the NLRP3 inflammasome were crucial for IL-1β secretion but not for IL-1β mRNA expression.

Furthermore, TLR4/MyD88/NF-κB signaling pathway was involved in PRRSV-induced expression of NLRP3 inflammasome components.

Together, our results deciphered the pathways leading from recognition of PRRSV to the production and release of IL-1β, providing a deeper knowledge of the mechanisms of PRRSV-induced inflammation responses.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Bi, Jing& Song, Shuang& Fang, Liurong& Wang, Dang& Jing, Huiyuan& Gao, Li…[et al.]. 2014. Porcine Reproductive and Respiratory Syndrome Virus Induces IL-1β Production Depending on TLR4MyD88 Pathway and NLRP3 Inflammasome in Primary Porcine Alveolar Macrophages. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-1043538

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Bi, Jing…[et al.]. Porcine Reproductive and Respiratory Syndrome Virus Induces IL-1β Production Depending on TLR4MyD88 Pathway and NLRP3 Inflammasome in Primary Porcine Alveolar Macrophages. Mediators of Inflammation No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-1043538

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Bi, Jing& Song, Shuang& Fang, Liurong& Wang, Dang& Jing, Huiyuan& Gao, Li…[et al.]. Porcine Reproductive and Respiratory Syndrome Virus Induces IL-1β Production Depending on TLR4MyD88 Pathway and NLRP3 Inflammasome in Primary Porcine Alveolar Macrophages. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-1043538

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1043538